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The isolated Gluconacetobacter sp. with accession number: KY996741 was assayed for evaluation of phytase activity. It could solubilise sodium phytate in the absence of soluble phosphate with the cells; however, the enzyme was not seen in cell free extract, to the best of their knowledge the intracellular phytase activities of Gluconacetobacter sp. was not reported previously. Also, the potential of in situ immobilisation of cells produced enzyme (/phytase producing bacteria) in bacterial cellulose was investigated and was studied by SEM and AFM. The results showed that the immobilised probiotic cells had the best activity of 1229 U/ml. The optimum temperature of the immobilised enzyme activity was at 45°C (5969 U/ml) and the immobilised phytase maintained 64% of its activities after two repeated cycles. The enzyme needs mild conditions for its activity and has a short life time and low stability and lost activities from 1229 to 500 U/ml during 30 days. However, it was showed that the addition of 1 ppm nano-ferric oxide particles could promote the phytase activities of immobilised cell from 500 U/ml to >1500 U/ml. This immobilised phytase producing cells on bacterial cellulose can be useful as food and/feed supplement for phytin removal.

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