Fig. 1. Schematic diagran optical system of spectroph grees. This arrangement gi' tion spectrum which is the verely influenced by scattei ment b permits the photo( light scattered angles up to 50 degrees. Arrangemen by Shibata et al. (3), is ec with respect to the collectio light and is experimental venient. A diffusing plate, hind the sample, causes a s resentative part of the lig to enter the photocell. Ar with identical diffusing pl opal glass placed behind th the blank vessel, was used the influence of scattering.The only spectrophotor which were different for th( ments were those for the ar the photocell response and 1 width (5 mg for a and 7 a wavelength of 690 mg; 1 wavelengths). Controls ir these differences did not results. As a further check ment and method, absorpti chlorophyll in acetone wx Sample (S) ioNX/s ?' . Diffusing Plate Fig. 1. Schematic diagran optical system of spectroph grees. This arrangement gi' tion spectrum which is the verely influenced by scattei ment b permits the photo( light scattered angles up to 50 degrees. Arrangemen by Shibata et al. (3), is ec with respect to the collectio light and is experimental venient. A diffusing plate, hind the sample, causes a s resentative part of the lig to enter the photocell. Ar with identical diffusing pl opal glass placed behind th the blank vessel, was used the influence of scattering. The only spectrophotor which were different for th( ments were those for the ar the photocell response and 1 width (5 mg for a and 7 a wavelength of 690 mg; 1 wavelengths). Controls ir these differences did not results. As a further check ment and method, absorpti chlorophyll in acetone wx 1.4 f <..,, d-,,,.in pl,t Q:0.3 r +s bse,kes,@se: 0. I WAVEtKEGTH(mp)--Fig. 2. Absorption spectra measured with Beckman D] spectrophotometer; arranger (shown in Fig. 1) were used. tion curves are reproductio: spectrophotometer recordin which was made in tripl: curve: scattering by Chlore grees. [From P. Latimer aI owitch (1) ] 30 1.4 f <..,, d-,,,.in pl,t Q:0.3 r +s bse,kes,@se: 0. I WAVEtKEGTH(mp)--Fig. 2. Absorption spectra measured with Beckman D] spectrophotometer; arranger (shown in Fig. 1) were used. tion curves are reproductio: spectrophotometer recordin which was made in tripl: curve: scattering by Chlore grees. [From P. Latimer aI owitch (1) ] 30 Pho toc el (P)with arrangements a and c. The shapes and positions of the bands on the two [2 curves were identical, but the absorption between the bands differed slightly, probably because of multiple reflections between the diffusing plate and the samb) ple vessel. Figure 2 shows the absorption curves of a Chlorella suspension (10-3 ml of cells per milliliter) obtained with ar-() rangements a and c and also the previ-_ _ ously reported scattering curve of this organism. The latter curve is for light scattered at 90 degrees but is qualitans of part of tively representative of light scattered at aotometer. most angles.Of particular interest are the differences of more than 10 mgx between the ves an absorp-positions of the major...