Thyrotropin receptor (TSHR), sodium iodide symporter (NIS), pendrin, and thyroid peroxidase (TPO) are essential for the uptake of iodine by follicular thyroid cells. The aim of this study was to establish immunohistochemistry (IHC) protocols for TSHR, NIS, pendrin, and TPO in canine tissues and characterize their expression in organoids derived from canine follicular cell thyroid carcinoma (FTC) and in the respective primary tumors. This constitutes a fundamental step to establish organoids as a model to study the uptake of iodine in canine FTC. Commercially available antibodies directed against human proteins were selected. Antibody specificity was confirmed by western blot using lysates of the HTori-3 human thyroid cell line and healthy canine thyroid gland. IHC was validated using HTori-3 cells and a set of canine normal tissues including healthy thyroid gland. The expression of TSHR, NIS, pendrin, and TPO was evaluated in 3 organoid lines derived from FTC and respective primary tumors. All 4 antibodies produced specific bands by western blot and cytoplasmic labeling in follicular cells by IHC in both human HTori-3 cells and canine thyroid gland. NIS also showed basolateral membrane immunolabeling in follicular cells. All 4 proteins were highly expressed in organoids derived from FTC. The expression was similar or higher compared to the primary tumors. The results of this study characterize organoids derived from canine FTC as a suitable in vitro model to investigate iodine uptake, opening new research possibilities in the field of canine thyroid cancer therapy.
Se habla de las traducciones de Aristóteles y Pseudo-Aristóteles en tierras gallegas o realizados por gallegos en la Edad Media; así como los problemas inherentes a toda operación de traducción en cualquier época y circunstancia. Nos centramos en Pedro Gallego, representante de la 3º generación de los traductores de la corte toledana de Alfonso X el Sabio, de quien fue amigo y confesor, aunque afincado en Murcia y obispo titular de la recién recuperada sede de Cartagena. Y pormenorizamos las distintas secuencias en el traslado de una de las obras capitales de Aristóteles, que tanto interesó a los árabes, del árabe al latín, el Liber de animalibus, compendio de diversos trabajos naturalistas y de biología, que traduce nuestro autor natural de Ortigueira (mariña lucense).
Exam of affinities and differences in the philosophy, of them qualified representatives of the Humanism of the XII and XIV centuries, John of Salisbury and Dante Alighieri respectively, through a compared study of Policraticus and De Monarchia; to the light of the secularization process of the culture and european thoughts at the end of the Medieval Age.
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