Integrated Ocean Drilling Program (IODP) Expedition 301, on the eastern flank of the Juan de Fuca Ridge, established part of a three-dimensional network of borehole observatories (Circulation Obviation Retrofit Kits [CORKs]) in the oceanic crust. These observatories are to be used to conduct active, multidisciplinary experiments over timescales of minutes to years and length scales of meters to kilometers. The complete experimental program will comprise two IODP expeditions (the first having been Expedition 301, the second to be scheduled), an offset seismic experiment, and long-term monitoring and crosshole testing carried out by submersible and remotely operated vehicle. During Expedition 301, we replaced a preexisting CORK observatory in Hole 1026B and created and instrumented new Holes U1301A and U1301B, which penetrate 108 and 320 m into basement, respectively. The borehole observatories deployed during Expedition 301 share some characteristics with systems deployed during the Ocean Drilling Program but also include many improved components and novel features.

[1] The Integrated Ocean Drilling Program (IODP) Hole 1301A on the eastern flank of Juan de Fuca Ridge was used in the first long-term deployment of microbial enrichment flow cells using osmotically driven pumps in a subseafloor borehole. Three novel osmotically driven colonization systems with unidirectional flow were deployed in the borehole and incubated for 4 years to determine the microbial colonization preferences for 12 minerals and glasses present in igneous rocks. Following recovery of the colonization systems, we measured cell density on the minerals and glasses by fluorescent staining and direct counting and found some significant differences between mineral samples. We also determined the abundance of mesophilic and thermophilic culturable organotrophs grown on marine R2A medium and identified isolates by partial 16S or 18S rDNA sequencing. We found that nine distinct phylotypes of culturable mesophilic oligotrophs were present on the minerals and glasses and that eight of the nine can reduce nitrate and oxidize iron. Fe(II)-rich olivine minerals had the highest density of total countable cells and culturable organotrophic mesophiles, as well as the only culturable organotrophic thermophiles. These results suggest that olivine (a common igneous mineral) in seawater-recharged ocean crust is capable of supporting microbial communities, that iron oxidation and nitrate reduction may be important physiological characteristics of ocean crust microbes, and that heterogeneously distributed minerals in marine igneous rocks likely influence the distribution of microbial communities in the ocean crust.

Integrated Ocean Drilling Program Expedition 301 was preceded during 2000 and 2002 by three surveys that helped to delineate seafloor and basement relief, sediment thickness, and the nature of ridge-flank hydrothermal conditions and processes on the eastern flank of the Juan de Fuca Ridge. These surveys generated swath map, seismic, and thermal data used to select locations for primary and secondary drilling targets, building from several decades of earlier work. We show compilations and examples of data from several characteristic settings in and around the Expedition 301 work area and use these observations to evaluate sedimentation patterns and thermal conditions in basement. There remain important unanswered questions in this area concerning fluid circulation within the upper oceanic crust, the magnitude of lithospheric heat input, the quantitative significance of advective heat loss from the crust, and relations between basement relief, sedimentation, and sediment alteration. These questions may be resolved through collection of a modest amount of additional data focusing on a few critical locations.

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We describe a new chamber-based benthic microbial fuel cell (BMFC) that incorporates a suspended, high surface area and semi-enclosed anode to improve performance. In Yaquina Bay, OR, two chambered BMFC prototypes generated current continuously for over 200 days. One BMFC was pumped intermittently, which produced power densities more than an order of magnitude greater than those achieved by previous BMFCs with single buried graphite-plate anodes. On average, the continuous power densities with pumping were 233 mW/m2 (2.3 W/m3); peak values were 380 mW/m2 (3.8 W/m3), and performance improved over the time of the deployments. Without pumping, high power densities could similarly be achieved after either BMFC was allowed to rest at open circuit. A third chambered BMFC with a 0.4 m2 footprint was deployed at a cold seep in Monterey Canyon, CA to test the new design in an environment with natural advection. The power density increased 5-fold (140 mW/m2 vs 28 mW/m2) when low-pressure check valves allowed unidirectional flow through the chamber.