BackgroundThe search for promising and renewable sources of carbohydrates for the production of biofuels and other biorenewables has been stimulated by an increase in global energy demand in the face of growing concern over greenhouse gas emissions and fuel security. In particular, interest has focused on non-food lignocellulosic biomass as a potential source of abundant and sustainable feedstock for biorefineries. Here we investigate the potential of three Brazilian grasses (Panicum maximum, Pennisetum purpureum and Brachiaria brizantha), as well as bark residues from the harvesting of two commercial Eucalyptus clones (E. grandis and E. grandis x urophylla) for biofuel production, and compare these to sugarcane bagasse. The effects of hot water, acid, alkaline and sulfite pretreatments (at increasing temperatures) on the chemical composition, morphology and saccharification yields of these different biomass types were evaluated.ResultsThe average yield (per hectare), availability and general composition of all five biomasses were compared. Compositional analyses indicate a high level of hemicellulose and lignin removal in all grass varieties (including sugarcane bagasse) after acid and alkaline pretreatment with increasing temperatures, whilst the biomasses pretreated with hot water or sulfite showed little variation from the control. For all biomasses, higher cellulose enrichment resulted from treatment with sodium hydroxide at 130°C. At 180°C, a decrease in cellulose content was observed, which is associated with high amorphous cellulose removal and 5-hydroxymethyl-furaldehyde production. Morphological analysis showed the effects of different pretreatments on the biomass surface, revealing a high production of microfibrillated cellulose on grass surfaces, after treatment with 1% sodium hydroxide at 130°C for 30 minutes. This may explain the higher hydrolysis yields resulting from these pretreatments, since these cellulosic nanoparticles can be easily accessed and cleaved by cellulases.ConclusionOur results show the potential of three Brazilian grasses with high productivity yields as valuable sources of carbohydrates for ethanol production and other biomaterials. Sodium hydroxide at 130°C was found to be the most effective pretreatment for enhanced saccharification yields. It was also efficient in the production of microfibrillated cellulose on grass surfaces, thereby revealing their potential as a source of natural fillers used for bionanocomposites production.

The narrow genetic variability of grasslands and the incidence of new biotic and abiotic stresses have motivated the selection of new Panicum maximum genotypes for use as forage for beef cattle in the Brazilian savannah. This study aimed to evaluate forage yield and nutritive value of P. maximum genotypes including 14 accessions (PM30 to PM43), four intraspecifi c hybrids (PM44 to PM47) and six cultivars (Aruana, Massai, Milênio, Mombaça, Tanzania and Vencedor), examining 24 genotypes over two years (2003 and 2004). Milênio cultivar was the genotype with the highest dry matter yield (DMY) in both years (18.4 t ha -1 and 20.9 t ha -1 , respectively) although it presented a high proportion of stems (~ 30 %). Genotypes that showed higher Leaf DMY in both years were the accession PM34 (14.7 t ha -1 ) and the hybrid PM46 (14.0 t ha -1 ), while Mombaça and Tanzania yielded 12.5 and 11.0 t ha -1 , respectively. Leaf organic matter digestibility and leaf DMY for PM40 and PM46 genotypes exceeded the mean (> 656 g kg -1and > 11.7 t ha -1 , respectively). For this reason, PM40 and PM46 can be considered promising P. maximum genotypes for use as forage for grazing systems in the Brazilian savannah. IntroductionThe neotropical savannah known as Cerrado, located in the center of the Brazilian territory, presents 49 million hectares of cultivated pastures (Sano et al., 2007). Even though they are quite well adapted to general environmental conditions, their narrow genetic variability makes these pastures vulnerable to novel biotic and abiotic stresses. Examples of this are the emergent sap-sucking insect well-known as the sugarcane spittlebug (Mahanarva spp.), and a syndrome called 'sudden death' that have reached large areas of Marandu grass (Brachiaria brizantha Hochst. ex A. Rich. Stapf.) pastures in the Northern Brazilian territory, with a complex diagnosis usually related to temporarily fl ooded areas (Auad et al., 2010;Caetano and Dias-Filho, 2008). To expand the genetic diversity with highly productive species and to decrease the susceptibility of Brazilian pastures to upcoming stresses, new grass genotypes have undergone assessment by Brazilian research institutions (Sousa et al., 2011, Pessim et al., 2010, Resende et al., 2004.As an alternative to Brachiaria spp., Guinea grass (Panicum maximum Jacq.) has been used for more intensive cattle production systems. It is recommended for regions where annual rainfall ranges from 800 to 1800 mm in well-drained soil and it requires medium to high soil fertility (Muir and Jank, 2004). Tanzania and Mombaça are the most planted Guinea grass cultivars in Brazil, both collected in Africa in the 1960s by ORSTOM (Offi ce de la Recherche Scientifi que et Technique d'OutreMer) and selected by EMBRAPA (Brazilian Agricultural Research Corporation) in the early 1990s. Currently, these two high-sized tufted grass cultivars are responsible for 10 % of the forage seed market in Brazil, but the incidence of the leaf fungus Bipolaris maydis (Nisik. Schoemaker) has reduced their deman...

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