A genome-wide survival analysis of 14,406 Alzheimer’s disease
(AD) cases and 25,849 controls identified eight previously reported AD risk loci
and fourteen novel loci associated with age at onset. LD score regression of 220
cell types implicated regulation of myeloid gene expression in AD risk. In
particular, the minor allele of rs1057233 (G), within the previously reported
CELF1 AD risk locus, showed association with delayed AD
onset and lower expression of SPI1 in monocytes and
macrophages. SPI1 encodes PU.1, a transcription factor critical
for myeloid cell development and function. AD heritability is enriched within
the PU.1 cistrome, implicating a myeloid PU.1 target gene network in AD.
Finally, experimentally altered PU.1 levels affect the expression of mouse
orthologs of many AD risk genes and the phagocytic activity of mouse microglial
cells. Our results suggest that lower SPI1 expression reduces
AD risk by regulating myeloid gene expression and cell function.
ehaviors related to self-regulation, such as substance use disorders or antisocial behaviors, have far-reaching consequences for affected individuals, their families, communities and society at large 1,2 . Collectively, this group of correlated traits are classified as externalizing 3 . Twin studies have demonstrated that externalizing liability is highly heritable (~80%) 4,5 . To date, however, no large-scale molecular genetic studies have utilized the extensive degree of genetic overlap among externalizing traits to aid gene discovery, as most studies have focused on individual disorders 6 . For many high-cost, high-risk behaviors with an externalizing component-opioid use disorder and suicide attempts 7 being salient examples-there are limited genotyped cases available for gene discovery 8,9 .A complementary strategy to the single-disease approach is to study the shared genetic architecture across traits in multivariate analyses, which boosts statistical power by pooling data across
A genome-wide survival analysis of 14,406 Alzheimer’s disease (AD) cases and 25,849 controls identified eight previously reported AD risk loci and fourteen novel loci associated with age at onset. LD score regression of 220 cell types implicated regulation of myeloid gene expression in AD risk. In particular, the minor allele of rs1057233 (G), within the previously reported CELF1 AD risk locus, showed association with delayed AD onset and lower expression of SPI1 in monocytes and macrophages. SPI1 encodes PU.1, a transcription factor critical for myeloid cell development and function. AD heritability is enriched within the PU.1 cistrome, implicating a myeloid PU.1 target gene network in AD. Finally, experimentally altered PU.1 levels affect the expression of mouse orthologs of many AD risk genes and the phagocytic activity of mouse microglial cells. Our results suggest that lower SPI1 expression reduces AD risk by regulating myeloid gene expression and cell function.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.