Background
The current study aims at evaluating the role of circulating or cell‐free long noncoding ribonucleic acid (lncRNA) growth arrest‐specific 5 (GAS5) in plaque psoriasis and at investigating its relationship with the presented clinical data.
Methods
This case‐control study was conducted on 180 subjects, divided into two main categories as follows: 90 cases with plaque psoriasis and 90 age‐ and sex‐matched healthy controls. Full history taking, thorough general examination, and full dermatological examination with determination of number and site of lesions were performed. Disease severity was assessed by Psoriasis Area Severity Index (PASI) score. Relative quantification of the expression level of cell‐free lncRNA (GAS5) was performed using real‐time polymerase chain reaction (RT‐PCR) technique.
Results
There was significant increase of GAS5 expression level in cases when compared with controls (U = 719.0, P < 0.001). Indeed, there was significant positive correlation between GAS5 and PASI score (r = 0.0.668, P < 0.001). Receiver operating characteristic (ROC) curve showed that GAS5 could identify patients from controls: GAS5 at a cut‐off value ≥0.31 provides a sensitivity of 95.56% and a specificity of 82.22%; at a cut‐off value ≥0.75, it can differentiate between mild and moderate cases, at a sensitivity of 77.78% and a specificity of 91.43%; at a cut‐off value ≥1.61, it can discriminate between moderate and severe cases, with a sensitivity of 71.43% and a specificity of 74.07%.
Conclusion
lncRNA GAS5 expression could be considered as a diagnostic marker of plaque psoriasis and indicator of its severity.
Background
About 30% of cases with psoriasis will suffer from psoriatic arthritis (PsA). Heritable element plays a role in PsA as different genes are involved. However, few genes are involved in both psoriasis and PsA. This study aimed to investigate the predictive value of Toll-like receptor (TLR) 4 and colony stimulating factor (CSF) 2 gene expression for early detection of axial spondyloarthritis in psoriatic patients.
Methods
This study included 200 subjects; 100 psoriatic patients, subdivided into two groups; Group 1: included 66 patients with plaque psoriasis without any articular complaint, and Group 2: included 44 patients with psoriatic arthritis. Group 3 included: 100 age and sex matched healthy controls. Laboratory assessment of TLR4 and CSF2 gene expression by real time polymerase chain reaction technique, and axial joint radiological assessment by Magnetic Resonance Imaging.
Results
There were significant increase of CSF2 and TLR4 gene expression levels in cases compared with controls (p < 0.001) for both. Additionally, a significant rise of CSF2 and TLR4 gene expression levels in cases with psoriatic arthritis compared to cases with psoriatic skin affection only (U = 2.45, p = 0.01, 3.34, p = 0.001 respectively. Receiver operating characteristic curve done for earlier detection of sub-clinical changes of axSpA regarding positive MRI results in cases with psoriasis and PsA respectively with P value < 0.001.
Conclusion
TLR4 and CSF2 gene expression have strong predictive value in early detection of axial SpA changes in asymptomatic and non-radiographic psoriatic patients which is equivalent and equal to the MRI predictive value.
Background: About 30% of cases with psoriasis will suffer from psoriatic arthritis (PsA).Heritable element plays a role in PsA as different genes are involved. However, few genes are involved in both psoriasis and PsA. This study aimed to investigate the predictive value of Toll-like receptor (TLR) 4 and colony stimulating factor (CSF) 2 gene expression for early detection of axial spondyloarthritis in psoriatic patients. Methods: This study included 200 subjects; 100 psoriatic patients, subdivided into two groups; Group 1: included 66 patients with plaque psoriasis without any articular complaint, and Group 2: included 44 patients with psoriatic arthritis. Group 3 included: 100 age and sex matched healthy controls. Laboratory assessment of TLR4 and CSF2 gene expression by real time polymerase chain reaction technique, and axial joint radiological assessment by Magnetic Resonance Imaging. Results: There were significant increase of CSF2 and TLR4 gene expression levels in cases compared with controls (p <0.001) for both. Additionally, a significant rise of CSF2 and TLR4 gene expression levels in cases with psoriatic arthritis compared to cases with psoriatic skin affection only (U=2.45, p= 0.01, 3.34, p= 0.001 respectively. Receiver operating characteristic curve done for earlier detection of sub-clinical changes of axSpA regarding positive MRI results in cases with psoriasis and PsA respectively with P value <0.001.
Conclusion: TLR4 and CSF2 gene expression have strong predictive value in early detectionof axial SpA changes in asymptomatic and non-radiographic psoriatic patients which is equivalent and equal to the MRI predictive value.
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