The use of fast and precise procedures for seed analysis is an important factor for making decisions at different stages of seed production. The aim of this study was to evaluate the occurrence of internal damage and associate morphological traits of pearl millet seeds with their physiological potential, and evaluate seed vigor using the Seed Vigor Imaging System (SVIS®) compared to other traditional vigor tests. Eight seed lots of the pearl millet cultivar ADR 300 were analyzed for germination, vigor (first count, accelerated aging, cold test, and SVIS®), internal morphology (X-ray), and morphological parameters (perimeter, area, and roundness). The X-ray test is effective in evaluating the internal morphology of pearl millet seeds, allowing identification of mechanical damage and of deteriorated and malformed tissues, making it possible to establish a relationship between the damage observed and the negative effect on germination. SVIS® analyses on two-day-old seedlings are a reliable method for assessing seed vigor of pearl millet. Parameters of perimeter and area of the seeds are related to their physiological potential.
RESUMO -A utilização de raios X em sementes é uma técnica eficiente na avaliação da morfologia interna de sementes de diversas espécies; trata-se de um método rápido, preciso e não destrutivo. Assim, a presente pesquisa teve o objetivo de avaliar a morfologia interna das sementes de sorgo, por meio de raios X, e relacioná-la com a germinação. Para tanto foram utilizadas sementes do híbrido 50A70, representado por dez lotes. As sementes radiografadas foram submetidas ao teste de germinação, de modo a relacionar a morfologia interna das sementes com as respectivas plântulas normais, anormais ou sementes mortas. Concluiu-se que o teste de raios X é eficiente na identificação de danos por deterioração de tecidos, danos mecânicos, danos por malformação e danos por insetos, possibilitando estabelecer a relação entre o dano observado e o prejuízo causado para a germinação. Palavras-chave:Sorghum bicolor, análise de imagens, germinação. X-RAY IN THE EVALUATION OF INTERNAL MORPHOLOGY OF SORGHUM SEEDSABSTRACT -The use of X-ray in seeds is an efficient technique for the evaluation of the internal seed morphology; it is considered a fast, accurate and non-destructive method. Thus, this study aimed to evaluate the internal morphology of sorghum seeds through X-ray. Therefore, seeds of hybrid 50A70 were used, represented by ten lots. The X-rayed seeds were submitted to germination test in order to associate the internal morphology of the seeds with normal or abnormal seedlings and dead seeds. The results allowed concluding that the X-ray test is effective in identifying damage tissue deterioration, mechanical damage, malformation damage and insect damage, enabling to establish a relationship between the observed damage and the negative effect on germination.
Companies have sought to improve, more and more, the quality control of the seeds produced, improving tests that evaluate the vigor of the seeds with greater speed and precision. The objective of the present work was to study the effects of imbibition time on the results of the electrical conductivity test to evaluate the vigor of millet seeds and to correlate with the other tests carried out. Six seeds lots of the ADR300 cultivar with different vigor levels were used. Germination, first germination count, accelerated ageing, emergence and emergence speed of seedlings in sand and electrical conductivity with variations in water volume were performed (50 And 75 cm 3 ), during the imbibition time of 2, 4, 6 and 8 hours, at 25 ºC. The time of 2 hours of the seed imbibition in 75 cm 3 in water proved to be efficient to evaluate the vigor of millet seeds in all lots tested.
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