The present study investigates the remodeling of gap junctional organization in relation to changes in anisotropic conduction properties in hypertrophied right ventricles (RVs) of rats with monocrotaline (MCT)-induced pulmonary hypertension. In contrast to controls that showed immunolocalization of connexin43 (Cx43) labeling largely confined to the intercalated disks, RV myocytes from MCT-treated rats showed dispersion of Cx43 labeling over the entire cell surface. The disorganization of Cx43 labeling became more pronounced with the progression of hypertrophy. Desmoplakin remained localized to the intercalated disks, as in controls. In RV tissues, the proportion of Cx43 label at the intercalated disk progressively decreased. Quantitative analysis of en face views of intercalated disks revealed a significant decrease in the disk gap junctional density in RV tissues of MCT-treated rats (control, 0.18 versus MCT-treated, 0.14 at 2 weeks; control, 0.16 versus MCT-treated, 0.11 at 4 weeks). Conduction velocity in RVs parallel to the fiber orientation was significantly lower (30.2% [n=9]) in MCT-treated rats at 4 weeks than in control rats, whereas there was no significant difference observed in the conduction velocity across the fiber orientation between control and MCT-treated rats. The anisotropic ratio of MCT-treated rats (1.38+/-0.10) was significantly lower than that of control rats (1.98+/-0.12). These results suggest that RV hypertrophy induced by pressure overload is associated with both disorganization of gap junction distribution and alteration of anisotropic conduction properties.
ap junctions are specialized membrane regions consisting of groups of channels that directly connect the cytoplasmic components of adjacent cells and enable intercellular communication with respect to the exchange of ions and small (<1 kDa) molecules. 1 Gap junctions are composed of transmembrane proteins that belong to the connexin family. The principal gap junctional protein expressed in ventricles of the mammalian heart is connexin43 (Cx43), although connexin40, connexin45 and connexin 30.2 (and its human ortholog, connexin31.9) are also expressed in other regions of the heart. [2][3][4] Remodeling of gap junction in the heart is an important feature of the structural substrates for conduction disturbance and arrhythmogenesis under various pathological conditions including myocardial ischemia, infarction and hypertrophy. [2][3][4][5][6][7][8] In our previous immunohistochemical studies on rats with pressure overload-induced ventricular hypertrophy, we have shown that the cellular distribution of gap junctions are altered; in normal ventricles Cx43 gap junctions are largely confined to the intercalated disks at the cell termini, but in hypertrophied ventricles, Cx43 gap junctions are displaced from the intercalated disks and widely distributed. 9,10 Cx43 is a phosphoprotein, and the phosphorylation/dephosphorylation of Cx43 plays important roles in the regulation of protein turnover dynamics (trafficking, plaque assembly, internalization and degradation) as well as channels gating properties. 1,[11][12][13][14] In the hearts subjected to acute ischemia, [15][16][17] and in non-ischemic heart failure, 18 as well as in dilated cardiomyopathy, 19 dephosphorylation of Cx43 was shown to play an important role in its disorganization and electrical uncoupling of ventricular cells under the pathological conditions. It is well known that ventricular hypertrophy is associated with the activation or inhibition of various types of protein kinases and/or phosphatases. We, therefore, hypothesized that phosphorylation/dephosphorylation of Cx43 could also be involved in the disorganization of Cx43 gap junctions in the pressure-overload ventricular hypertrophy.In the present study, we have investigated the expression and distribution of Cx43 and its phosphorylation state in hypertrophied ventricles of rats with monocrotaline (MCT)-induced pulmonary hypertension by immunoconfocal and electron microscopy, as well as immunoblotting using isoform-specific antibodies. Background Altered expression and distribution of gap junctions might provide substrates for abnormal conduction and arrhythmogenesis in the heart, but little is known about the regulation of gap junctions under pathological conditions. The organization and phosphorylation state of connexin43 (Cx43) in ventricular hypertrophy will be investigated.
Methods and ResultsRight ventricular (RV) hypertrophy was induced in rats by treatment with monocrotaline. Subcellular Cx43 distribution was assessed by immunoconfocal and electron microscopy. Immunolabeling of Cx43 was conf...
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