The aim of this in-vivo study was to compare total protein and four key salivary proteins present in the acquired enamel pellicle (AEP) on eroded and non-eroded surfaces in participants with erosive tooth wear. Participants with erosive tooth wear of dietary non-intrinsic origin, present on the occlusal surfaces of the lower first molars and an unaffected posterior occlusal surface in the same quadrant were recruited from restorative dental clinics at King’s College London Dental Institute (n = 29, REC ref 14/EM/1171). Following removal of the salivary film, AEP samples were collected from the eroded occlusal surfaces (EP, n = 29) and the non-eroded occlusal surfaces (NP, n = 29) using 0.5% sodium dodecyl sulfate (SDS) soaked filter papers. Total protein concentration was analysed using bicinchoninic acid assay (BCA). Protein fractions were separated using SDS-PAGE and immunoblotted against: mucin5b, albumin, carbonic anhydrase VI (CA VI) and statherin antibodies. Amounts were quantified using ImageLab software against purified protein standards of known concentration. ANOVA followed by paired t-test and Wilcoxon’s matched-pair signed-rank test were used to test statistical significance. The difference was considered to be significant at a P value < 0.05. The total protein on eroded surfaces was significantly lower compared to the total protein on non-eroded surfaces [0.41mg/mL (0.04) and 0.61 mg/mL (0.11)] respectively (p< 0.05). The median (min, max) amount of statherin was also significantly lower on eroded occlusal surfaces [84.1 (20.0, 221.8) ng] compared to AEP from non-eroded teeth in the same subjects [97.1(30.0, 755.6) ng] (p = 0.002). No statistical differences were observed for mucin 5b, albumin or CA VI. The total protein and statherin in the in-vivo AEP were different between eroded and non-eroded tooth surfaces of the same patient.
Flexural strength (FS) and translucency (Contrast Ratio-CR) of three different factory crystallized silica-based glass ceramics, Celtra Duo (CD), N!ce (NI) and Li-Si Block, a lithium disilicate, IPS e.max CAD (LD), and a leucite-reinforced feldspathic ceramic, Empress CAD (EM), in two different translucencies (HT and LT) for use in chairside dental restorations have been compared. CAD blocks of the materials were cut into beams and tiles and processed following manufacturers’ instructions. The beams were tested (3-PBT) to determine flexural strength, Weibull characteristic strength, and Weibull modulus; and tiles were tested to determine CR. All data were statistically analyzed. In addition, SEM analysis of the materials was performed. Differences in flexural strength (FS) and translucency (CR) between the materials were found to be statistically significant. FS decreased as follows (MPa): LDHT 350.88 ± 19.77 (a) = LDLT 343.57 ± 18.48 (a) > LSLT 202.15 ± 17.41 (b) = LSHT 196.93 ± 8.87 > NIHT 186.69 ± 13.06 (c) = CDLT 184.73 ± 13.63 (c) = CDHT 174.15 ± 21.76 (c) = NILT 172.12 ± 11.98 (c) > EMHT 131.16 ± 13.33 (e) = EMLT 127.65 ± 11.09. CR decreased as follows (mean ± sd): CDLT 74.1 ± 1.1 (a); LSLT 74.0 ± 1.1 (ab); NILT 73.3 ± 0.8 (ab); EMLT 73.0 ± 1.5 (ab); NIHT 72.4 ± 1.0 (bc); LDLT 71.3 ± 1.1 (bc); LTHT 65.2 ± 0.9 (de); LSHT 63.8 ± 1.1 (def); EMHT 636 ± 1.2 (ef); CDHT 62.2 ± 0.8 (f). Our findings show that factory-crystallized lithium silicate glass ceramics fulfill ISO standards for Classes 1 and 2. Therefore, they can be considered viable alternatives to produce single-unit restorations with a chairside procedure not requiring thermal treatment.
Erosive wear undermines the structural properties of enamel resulting in irreversible enamel loss. A thin protein layer formed from natural saliva on tooth surfaces, acquired enamel pellicle (AEP), protects against erosive wear. The exact components in saliva responsible for such protection are not yet known. We prepared three solutions containing different components: proteins and ions [natural saliva (NS)], minerals with no proteins [artificial saliva (AS)] and neither proteins nor ions [deionised water (DW)]. To assess the protection of the three solutions against citric acid enamel erosion, enamel specimens were immersed in the corresponding solution for 24 h. All specimens were then exposed to five erosion cycles, each consisted of a further 30 min immersion in the same solution followed by 10-min erosion. Mean step height using a non-contacting profilometer, mean surface microhardness (SMH) using Knoop microhardness tester (final SMH), and roughness and 2D profiles using atomic force microscopy were measured after five cycles. The final SMH values were compared to the starting values (after 24 hr). NS group had significantly less tissue loss but greater SMH change (P < 0.0001) than AS and DW groups. Specimens in NS were softer and rougher (P < 0.001) but less eroded than specimens in AS and DW.
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