Background
Antioxidant agents have positive effects on wound healing process and on the other hand probiotics also have great antioxidation or oxidation-resistance properties.
Methods
In this study, we investigated the effects of Lactobacillus reuteri derived ointment as a probiotic bacterium on the wound healing process using a full-thickness rat skin model. The wound diameter and contraction percent measurement, histopathological examination, and myeloperoxidase (MPO) enzyme activity evaluation were carried out during the period of study.
Results
Amount of deposit collagen was significantly increased; epithelization speed and epidermis production were also observed in the ointment-treated group, while the inflammation rate was also significantly lower than the control group. In addition, a significant reduction in the activity of enzyme MPO was also detected in the ointment-treated group.
Conclusions
According to the results, probiotic ointment effectively accelerates the wound healing process and it can be used as a promising drug for wound dressing application.
We aimed to explore the lncRNA–miR–mRNA network in response to Lactobacillusacidophilus (L. acidophilus) consumption in rectal cancer patients. The candidate miRs were first taken from the GEO and TCGA databases. We constructed the lncRNA–miR–mRNA network using the high-throughput sequencing data. At last, we created a heatmap based on the experimental data to show the possible correlation of the selected targets. The expression levels of selected targets were measured in the samples of 107 rectal cancer patients undergoing placebo and probiotic consumption and 10 noncancerous subjects using Real-Time PCR. Our analysis revealed a group of differentially expressed 12 miRs and 11 lncRNAs, and 12 genes in rectal cancer patients. A significant expression increase of the selected tumor suppressor miRs, lncRNAs, and genes and a substantial expression decrease of the selected oncomiRs, onco-lncRNAs, and oncogenes were obtained after the probiotic consumption compared to the placebo group. There is a strong correlation between some network components, including miR-133b and IGF1 gene, miR-548ac and MSH2 gene, and miR-21 and SMAD4 gene. In rectal cancer patients, L.acidophilus consumption was associated with improved expression of the lncRNA–miR–mRNA network, which may provide novel monitoring and therapeutic approaches.
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