Voltage-gated sodium channels underlie the rapid regenerative upstroke of action potentials and are modulated by cytoplasmic calcium ions through a poorly understood mechanism. We describe the 1.35 Å crystal structure of Ca 2+ -bound calmodulin (Ca 2+ /CaM) in complex with the inactivation gate (DIII-IV linker) of the cardiac sodium channel (Na V 1.5). The complex harbors the positions of five disease mutations involved with long Q-T type 3 and Brugada syndromes. In conjunction with isothermal titration calorimetry, we identify unique inactivation-gate mutations that enhance or diminish Ca 2+ /CaM binding, which, in turn, sensitize or abolish Ca 2+ regulation of full-length channels in electrophysiological experiments. Additional biochemical experiments support a model whereby a single Ca 2+ /CaM bridges the C-terminal IQ motif to the DIII-IV linker via individual N and C lobes, respectively. The data suggest that Ca 2+ /CaM destabilizes binding of the inactivation gate to its receptor, thus biasing inactivation toward more depolarized potentials.crystallography | patch-clamp electrophysiology | structural biology | cardiac arrhythmia V oltage-gated sodium channels (Na V s) support excitability in the cardiovascular and nervous systems, where they contribute to the rhythm and rate of action potentials. These large (∼220-kDa) transmembrane protein complexes are expressed at a high density in excitable cells, where they conduct large macroscopic inward sodium currents. These channels are exquisitely sensitive to subtle changes in the transmembrane potential, and modest alterations in channel gating can fine-tune or disorder electrical signaling at the organ and systemic level. The α-subunit of the channel contains cytoplasmic amino and carboxyl termini and is composed of four homologous transmembrane domains (DI-DIV) that are connected by intracellular linkers. Each domain contains voltage-sensing (S1-S4) and pore-forming (S5 and S6) domains that form the selectivity filter and putative activation gates. A crystal structure of a bacterial Na V was recently described (1) showing a similar overall fold compared with potassium channels. However, this bacterial variant is homotetrameric, and seems to lack a conserved fast-inactivation mechanism. As such, it has no homology with several relevant domains in mammalian Na V channels, and no crystal structure of any eukaryotic Na V region has yet been reported.Calcium ions (Ca 2+ ) are universal second messengers, and in the heart they form the electrochemical link between plasma membrane depolarization and myocyte contraction. Consequently, their cytoplasmic levels oscillate between nanomolar and micromolar levels with each excitation-contraction cycle (2). Sodium channel steady-state inactivation, a process that controls channel availability at a given transmembrane potential, is modulated through interactions with Ca 2+ and calmodulin (CaM) (3-10). The mechanistic details of Ca 2+ modulation of sodium channel inactivation are sparse, but the C-terminal region of the cha...
Synthetic biology and metabolic engineering seek to re-engineer microbes into “living foundries” for the production of high value chemicals. Through a “design-build-test” cycle paradigm, massive libraries of genetically engineered microbes can be constructed and tested for metabolite overproduction and secretion. However, library generation capacity outpaces the rate of high-throughput testing and screening. Well plate assays are flexible but with limited throughput, whereas droplet microfluidic techniques are ultrahigh-throughput but require a custom assay for each target. Here we present RNA-aptamers-in-droplets (RAPID), a method that greatly expands the generality of ultrahigh-throughput microfluidic screening. Using aptamers, we transduce extracellular product titer into fluorescence, allowing ultrahigh-throughput screening of millions of variants. We demonstrate the RAPID approach by enhancing production of tyrosine and secretion of a recombinant protein in Saccharomyces cerevisiae by up to 28- and 3-fold, respectively. Aptamers-in-droplets affords a general approach for evolving microbes to synthesize and secrete value-added chemicals.
A large number of toxins from sea anemone have been previously shown to modulate voltage-gated Naþ channel (Nav) function. The aim of this study was to test whether APETx2, at concentrations used to block heterotrimeric ASIC3 containing channels, affects Nav function in sensory neurons. The effect of APETx2 on Nav function was studied using the whole-cell patch-clamp technique on acutely dissociated small-diameter rat dorsal root ganglion (DRG) neurons. In our study, APETx2 inhibited the tetrodotoxin (TTX)-resistant Nav1.8 currents of DRG neurons in a concentration-dependent manner with an IC50 of 3 mM. TTX-sensitive currents were inhibited to a smaller extent. The observed inhibition of Nav1.8 currents is due to a rightward shift in the voltage dependence of activation, and a reduction of the maximal macroscopic conductance. In current-clamp experiments in DRG neurons the number of action potentials induced by injection of a current ramp was reduced by APETx2. APETx2 inhibits, in addition to ASIC3, Nav1.8 channels at concentrations used in in vivo studies. The limited specificity of this toxin should be taken into account when using APETx2 as a pharmacological tool. Its dual action will be an advantage for the use of APETx2 or its derivatives as analgesic drugs.
Voltage-gated sodium channels maintain the electrical cadence and stability of neurons and muscle cells by selectively controlling the transmembrane passage of their namesake ion. The degree to which these channels contribute to cellular excitability can be managed therapeutically or fine-tuned by endogenous ligands. Intracellular calcium, for instance, modulates sodium channel inactivation, the process by which sodium conductance is negatively regulated. We explored the molecular basis for this effect by investigating the interaction between the ubiquitous calcium binding protein calmodulin (CaM) and the putative sodium channel inactivation gate composed of the cytosolic linker between homologous channel domains III and IV (DIII-IV). Experiments using isothermal titration calorimetry show that CaM binds to a novel double tyrosine motif in the center of the DIII-IV linker in a calcium-dependent manner, N-terminal to a region previously reported to be a CaM binding site. An alanine scan of aromatic residues in recombinant DIII-DIV linker peptides shows that whereas multiple side chains contribute to CaM binding, two tyrosines (Tyr 1494 and Tyr 1495 ) play a crucial role in binding the CaM C-lobe. The functional relevance of these observations was then ascertained through electrophysiological measurement of sodium channel inactivation gating in the presence and absence of calcium. Experiments on patch-clamped transfected tsA201 cells show that only the Y1494A mutation of the five sites tested renders sodium channel steady-state inactivation insensitive to cytosolic calcium. The results demonstrate that calcium-dependent calmodulin binding to the sodium channel inactivation gate double tyrosine motif is required for calcium regulation of the cardiac sodium channel.
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