Cyclin-dependent kinases (CDKs) are involved in many crucial processes, such as cell cycle and transcription, as well as communication, metabolism, and apoptosis. The kinases are organized in a pathway to ensure that, during cell division, each cell accurately replicates its DNA, and ensure its segregation equally between the two daughter cells. Deregulation of any of the stages of the cell cycle or transcription leads to apoptosis but, if uncorrected, can result in a series of diseases, such as cancer, neurodegenerative diseases (Alzheimer’s or Parkinson’s disease), and stroke. This review presents the current state of knowledge about the characteristics of cyclin-dependent kinases as potential pharmacological targets.

GPR18, still considered an orphan receptor, may respond to endocannabinoids, whose canonical receptors are CB and CB. GPR18 and CB receptors share a role in peripheral immune response regulation and are co-expressed in microglia, which are immunocompetent cells in the central nervous system (CNS). We aimed at identifying heteroreceptor complexes formed by GPR18 and CBR or CBR in resting and activated microglia. Receptor-receptor interaction was assessed using energy-transfer approaches, and receptor function by determining cAMP levels and ERK1/2 phosphorylation in heterologous cells and primary cultures of microglia. Heteroreceptor identification in primary cultures of microglia was achieved by in situ proximity ligation assays. Energy transfer results showed interaction of GPR18 with CBR but not with CBR. CB-GPR18 heteroreceptor complexes displayed particular functional properties (heteromer prints) often consisting of negative cross-talk (activation of one receptor reduces signaling arising from the partner receptor) and cross-antagonism (the response of one of the receptors is blocked by a selective antagonist of the partner receptor). Activated microglia showed the heteromer print (negative cross-talk and bidirectional cross-antagonism) and increased expression of CBR and GPR18. Due to the important role of CBR in neuroprotection, we further investigated heteroreceptor occurrence in primary cultures of microglia from transgenic mice overexpressing human APP, an Alzheimer's disease model. Microglial cells from transgenic mice showed the heteromer print and functional interactions that were similar to those found in cells from wild-type animals that were activated by treatment with lipopolysaccharide and interferon-γ. Our results suggest that GPR18 and its heteromers may play important roles in neurodegenerative processes.

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Introduction and objective. A nationwide survey, carried out in Poland in 2013, showed that 42% of an examined group of doctors reported occupational burnout syndrome (OBS). The phenomenon of OBS among medical personnel shows a relationship with perceived stress scale (PSS) scores. The aim of the study was to estimate the prevalence of OBS in a group of Polish doctors, and the relationship with selected risk factors and personal resources. Materials and method. A cross-sectional study using quantitative methodology was used with the application of a questionnaire method and correlation design. Questionnaires were administered to 318 doctors (42 different specialties) working in medical facilities in Poland. All participants in the study completed two standardized questionnaires: Link Burnout Questionnaire (LBQ), Perceived Stress Scale (PSS), and responded to the questions on a proprietary questionnaire. ANOVA variance analysis and analysis of regression was performed. Results. The results of the measurement of four aspects of occupational burnout: psychophysical exhaustion (PE), commitment to relationships with patients (CP), effectiveness in performed work (EW), and existential expectations (EE), were in the medium and high levels range. Every second medical doctor who participated in the research declared a high degree of occupational burnout in each of the aspects of OBS. The high level of PSS scores, the large number of duties per month, the short work experience (years of employment) and the low number of holiday leaves, were the predictors of occupational burnout in the group of doctors taking part in the study. Conclusions. The prevalence of the signs of OBS among Polish doctors is consistent with the results of research in other countries. Failures in the interventions taken to reduce stress seem to co-exist with the severity of signs of burnout.

Available data support the proposition that the examined compounds with adenosine A receptor affinity may be an interesting target for the development of antidepressant and/or anxiolytic agents.

A b s t r a c tBovine tuberculosis is an infectious disease that occurs in many species of both domestic and wild animals, as well as those held in capti vity. The etiological factor is the acid resistant bacillus (Mycobacterium bovis or Mycobacterium caprae), which is characterized by the major pathogenicity among mycobacteria belonging to the Mycobacterium tuberculosis complex. The material from 8 antelopes from the zoo, suspected for tuberculosis were examined, and M. bovis strains were isolated from 6 of them. The spoligotyping method showing spoligo pattern 676763777777600. In Poland, this spoligotype has not been observed so far.K e y w o r d s: Mycobacterium bovis, antelopes, MTBC, public health, zoo

Background: The Depression Anxiety Stress Scales (DASS) are designed to identify quickly and differentiate between the symptoms of depression and anxiety in the non-clinical population. Different versions (original and short) were validated in many cultures. Nevertheless, there are no data of factorial validity of the different versions of this scale in Polish culture. Thus, the aim of this study was to evaluate the factor structure using confirmatory factor analysis (CFA) and internal consistency of DASS-42 (original version) and two short versions (DASS-21 items and DASS-12 items) in the Polish population.Methods: The DASS-42 was administered to a non-clinical sample, broadly representative of the general Polish adult population (n = 1,021) in terms of demographic variables. The DASS-21 and DASS-12 version used in this study comprise seven and four items from each of the following corresponding three subscales of the Polish version of DASS-42.Results: There were two models that fitted best for DASS-42: (a) modified three correlated factors (depression, anxiety, and stress) with cross-loadings and (b) second order (general factor of psychological distress) and three factors with cross-loadings. There were also two models that fitted best for DASS-21 and DASS-12: (a) modified three correlated factors (depression, anxiety, and stress) and (b) second order (general factor of psychological distress) and three factors.Conclusions: All three versions of DASS appear to have an acceptable factorial structure. However, the shorter versions (DASS-21 and DASS-12) may be more feasible to use in general medical practice and also be less burdensome to participants.

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