Genomic characterization of
Furfurilactobacillus rossiae
revealed that strains which were previously identified as
F. rossiae
are genetically heterogeneous. The 16S rRNA gene sequences of strains FUA3430, FUA3583, C5, FUA3115 and FUA3119, were 99.6 % identical to
F. rossiae
but the core genome analysis revealed that these strains share less than 93 % average nucleotide identity (ANI) with the
F. rossiae
type strain DSM 15814T. Because the ANI value is below the threshold for delineation of bacterial species, we propose the novel species Furfurilactobacillus milii sp. nov. with the type strain FUA3430T (=DSM 113338T=LMG 32478T). Strains of F. milii have smaller genomes than
F. rossiae
, lack the pdu-cbi-cob-hem cluster which is responsible for 1,2-propanediol utilization in
F. rossiae
, and lack genes involved in ethanolamine utilization. Two strains of the novel species (FUA3430T and FUA3583) were compared to
F. rossiae
FUA3214. Analysis of the cellular fatty acid composition and metabolite analysis did not reveal significant differences between F. milii sp. nov. and
F. rossiae
FUA3124. Although the growth requirements with respect to temperature and pH were very similar, only the strain of
F. rossiae
utilized melibiose and d-xylose. Morphological differences were also seen in the colony and cell size of the novel compared to
F. rossiae
.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.