We describe here a nonisotopic immunoassay, based on particle-counting technology, for the determination of urinary albumin. The assay takes only 35 min and has been fully automated on the IMPACT (Acade Diagnostic Systems, Brussels, Belgium) machine. The system measures albumin within a linear range between 6.25 and 50 mg/L and has a detection limit of 0.4 mg/L. Analytical recoveries at three concentrations ranged between 96% and 102%. Within-run precision ranged from 1.6% to 9.5%. The method was compared with a commercial nephelometric immunoassay system and a correlation coefficient of 0.996 was found for 216 urine samples. No antigen excess affects the shape of the curve in our system, whereas in nephelometry a 3 g/L solution of albumin starts to decrease the dose-response curve.
Summary.
Two unrelated children of Western European ancestry, originating from the North Flemish part of Belgium were found to have a deficiency of red‐cell glucose‐6‐phosphate dehydrogenase (D‐glucose‐6‐phosphate: NADP oxidoreductase, G6PD) activity, with chronic non‐spherocytic haemolytic disease. The first hemizygous propositus exhibited subsequent episodes of haemolytic jaundice precipitated by upper respiratory infections. The second hemizygous propositus had neonatal hyperbilirubinaemia and later chronic haemolytic disease enhanced by febrile upper respiratory tract infections.
Characterization of the enzyme of the first case revealed that it was a variant which had not been previously reported and it was tentatively named G6PD Kessel‐Lo.
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