Due to certain differences in terms of molecular structure, isoquinoline alkaloids from Chelidonium majus engage in various biological activities. Apart from their well-documented antimicrobial potential, some phenanthridine and protoberberine derivatives as well as C. majus extract present with anti-inflammatory and cytotoxic effects. In this study, the LC–MS/MS method was used to determine alkaloids, phenolic acids, carboxylic acids, and hydroxybenzoic acids. We investigated five individually tested alkaloids (coptisine, berberine, chelidonine, chelerythrine, and sanguinarine) as well as C. majus root extract for their effect on the secretion of IL-1β, IL-8, and TNF-α in human polymorphonuclear leukocytes (neutrophils). Berberine, chelidonine, and chelerythrine significantly decreased the secretion of TNF-α in a concentration-dependent manner. Sanguinarine was found to be the most potent inhibitor of IL-1β secretion. However, the overproduction of IL-8 and TNF-α and a high cytotoxicity for these compounds were observed. Coptisine was highly cytotoxic and slightly decreased the secretion of the studied cytokines. The extract (1.25–12.5 μg/mL) increased cytokine secretion in a concentration-dependent manner, but an increase in cytotoxicity was also noted. The alkaloids were active at very low concentrations (0.625–2.5 μM), but their potential cytotoxic effects, except for chelidonine and chelerythrine, should not be ignored.
Abstract:The aim of performed investigation was to evaluate the influence of changes in herbicide application system on herbicide residues in soil and sugar beet roots. Chemical weed control in sugar beet was carried out by herbicides that included substances such as phenmedipham, desmedipham, ethofumesate, metamitron, triflusulfuron and surfactant adjuvant applied in three different systems: two times application at bare soil (preemergence) and postemergence application (weeds in phase of 2-4 leaves) -system "A", 3 times split, postemergence application (full dose of herbicide mixture) -system "B" and 4 times application at 7 to 10 day intervals starting at the beginning of weed emergence -system "C". Samples of soil and roots of sugar beet were taken at the day of lifting.Herbicide residues were analysed using HPLC with UV-detection. At lifting time, in soil samples, where herbicides were applied in system "A", the residues of metamitron amounted from 0.0097 to 0.0132 mg/kg. Sum of all detected residues of applied substances amounted 0.0341-0.0458 mg/kg. In sugar beet root samples, the residues amounted to respectively, 0.0049-0.0064 and 0.0136-0.0247 mg/kg. The application of herbicides in "B" and "C" systems caused a significant decrease of residues by about 50% (system "B") and 65% (system "C") on average, in comparison with results obtained for herbicide application in "A" system. Residues of active substances determined in roots of sugar beet did not exceed acceptable limits (MRLs).
The purpose of the work was to analyse metazachlor contamination of the soil and metazachlor contamination of rape seeds. Monitoring tests were carried out during the 2010-2012 time period, on winter and spring oilseed rape fields located in south-western Poland. Soil and seed samples were collected at harvest time. The determination of metazachlor residues was conducted using gas chromatography with electron capture detection (GC/ECD). Based on the analysis of a total of 59 soil samples and 59 rape seed samples, metazachlor residue was detected in 45% of the soil samples of winter oilseed rape and in 71% of the soil samples of spring oilseed rape. Metazachlor contamination of rape seed was detected in 29% of winter rape samples and in 53% of spring rape samples. The concentration of assayed residue did not exceed 0.0005-0.0102 mg/kg. There were significantly higher amounts of metazachlor residue determined for the soil and seed samples of spring oilseed rape. None of the analyzed samples of oilseed rape seed showed a residue content exceeding the Maximum Residue Level (MRL).
The antimicrobial properties of herbs from Papaveraceae have been used in medicine for centuries. Nevertheless, mutual relationships between the individual bioactive substances contained in these plants remain poorly elucidated. In this work, phytochemical composition of extracts from the aerial and underground parts of five Papaveraceae species (Chelidonium majus L., Corydalis cava (L.) Schweigg. and Körte, C. cheilanthifolia Hemsl., C. pumila (Host) Rchb., and Fumaria vaillantii Loisel.) were examined using LC-ESI-MS/MS with a triple quadrupole analyzer. Large differences in the quality and quantity of all analyzed compounds were observed between species of different genera and also within one genus. Two groups of metabolites predominated in the phytochemical profiles. These were isoquinoline alkaloids and, in smaller amounts, non-phenolic carboxylic acids and phenolic compounds. In aerial and underground parts, 22 and 20 compounds were detected, respectively. These included: seven isoquinoline alkaloids: protopine, allocryptopine, coptisine, berberine, chelidonine, sanguinarine, and chelerythrine; five of their derivatives as well as non-alkaloids: malic acid, trans-aconitic acid, quinic acid, salicylic acid, trans-caffeic acid, p-coumaric acid, chlorogenic acid, quercetin, and kaempferol; and vanillin. The aerial parts were much richer in phenolic compounds regardless of the plant species. Characterized extracts were studied for their antimicrobial potential against planktonic and biofilm-producing cells of S. aureus, P. aeruginosa, and C. albicans. The impact of the extracts on cellular metabolic activity and biofilm biomass production was evaluated. Moreover, the antimicrobial activity of the extracts introduced to the polymeric carrier made of bacterial cellulose was assessed. Extracts of C. cheilanthifolia were found to be the most effective against all tested human pathogens. Multiple regression tests indicated a high antimicrobial impact of quercetin in extracts of aerial parts against planktonic cells of S. aureus, P. aeruginosa, and C. albicans, and no direct correlation between the composition of other bioactive substances and the results of antimicrobial activity were found. Conclusively, further investigations are required to identify the relations between recognized and unrecognized compounds within extracts and their biological properties.
SummaryThe aim of the studies was to evaluate the herbicidal contaminations in sugar beet roots. Monitoring tests were carried out during 2010-2012 on sugar beet plantation located in South-Western Poland. The samples of sugar beet roots were collected at harvest time. The determination of fenmedipham, desmedipham, ethofumesate, metamitrom, chloridazon and lenacil residues were conducted using the high performance liquid chromatography with ultraviolet detection (HPLC/UV -high-performance liquid chromatography with ultraviolet). Monitoring studies showed that 56% of tested root samples did not contain detectable residues of tested herbicides. The residues of herbicide determined in 44% of samples ranged from 0.0004 to 0.0096 mg/kg. The concentration of residues detected in tested root samples did not exceed the maximum residue levels (MRLs).Key words: herbicide; sugar beet; residues monitoring; MRLs StreszczenieCelem badań była ocena zanieczyszczeń herbicydowych w korzeniach buraka cukrowego. Badania monitoringowe prowadzono w latach 2010-2012 na plantacjach buraka cukrowego, zlokalizowanych w południowo-zachodniej Polsce. Próbki korzeni do analiz pobierano przed zbiorem buraka. Oznaczanie pozostałości: fenmedifamu, desmedifamu, etofumesatu, metamitronu, chlorydazonu i lenacylu wykonano techniką chromatografii cieczowej (HPLC/UV -high-performance liquid chromatography with ultraviolet). Na podstawie przeprowadzonych badań monitoringowych można stwierdzić, że w 56% próbek korzeni nie stwierdzono oznaczalnych pozostałości herbicydów. Pozostałości wykryte w 44% próbek wahały się w zakresie 0,0004-0,0096 mg/kg. W analizowanych próbkach korzeni buraka nie stwierdzono pozostałości, których stężenie przekraczało najwyższe dopuszczalne poziomy pozostałości (NDP).
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