Supplementation of the diet with 3.2 g EPA plus DHA per day markedly alters plasma phospholipid and PBMC fatty acid compositions. The lack of effect of FO upon PBMC functions may relate to the level of alpha-tocopherol included in the supplements.

Muscle function often becomes progressively more compromised in children with spastic cerebral palsy, leading to reduced mobility. This study aimed to examine the role that muscle connective tissue plays in this process. Severity of spasticity as determined by a range of clinical measures was assessed in 26 children (14 males 12 females; age range 4 to 17 years) with either diplegic or quadriplegic cerebral palsy (CP). Muscle biopsies from the vastus laeralis muscle were obtained for biomedical and histological analysis during orthopaedic surgery as part of the child's ongoing care. Total collagen was quantified by hydroxyproline determination. Two clinical measures of severity, Modified Ashworth Scale and Balance, were shown to have a highly significant correlation with collagen content, and Ambulatory Status, Clonus, and Selective Muscle Control all showed positive trends. Collagen I accumulated in spastic muscle's endomysium which appeared to be thickened, and fibrotic regions with sparse muscle fibres were evident in more severe cases. This suggests that collagen may be involved in increases in muscle stiffness observed in spasticity. Once developed, these changes are essentially irreversible and we suggest that future treatments should consider including prevention of muscle fibrosis.

In this study the cross-sectional area (in n = 14 female controls, 15 male controls, 11 female patients with schizophrenia, 15 male patients with schizophrenia) and fibre composition (in n = 11 female controls, 10 male controls, 10 female patients with schizophrenia, 10 male patients with schizophrenia) of the corpus callosum in post-mortem control and schizophrenic brains was examined. A gender x diagnosis interaction (P = 0.005) was seen in the density of axons in all regions of the corpus callosum except the posterior midbody and splenium. Amongst controls, females had greater density than males; in patients with schizophrenia this difference was reversed. A reduction in the total number of fibres in all regions of the corpus callosum except the rostrum was observed in female schizophrenic patients (P = 0.006; when controlling for brain weight, P = 0.053). A trend towards a reduced cross-sectional area of the corpus callosum was seen in schizophrenia (P = 0.098); however, this is likely to be no more than a reflection of an overall reduction in brain size. With age, all subregions of the corpus callosum except the rostrum showed a significant reduction in cross-sectional area (P = 0.018) and total fibre number (P = 0.002). These findings suggest that in schizophrenia there is a subtle and gender-dependent alteration in the forebrain commissures that may relate to the deviations in asymmetry seen in other studies, but the precise anatomical explanation remains obscure.

The development of a new parental self-report questionnaire, the Parental Interactive Bedtime Behaviour Scale (PIBBS) is described. The PIBBS was designed to capture a wide range of parental behaviours used to settle infants off to sleep. The commonest behaviours employed were giving a feed, talking softly to the child, cuddling in the arms, and stroking. A factor analysis revealed five settling strategies; 'active physical comforting' (e.g. cuddling in arms); 'encouraging infant autonomy' (e.g. leaving to cry); 'movement' (e.g. car rides), 'passive physical comforting' (e.g. standing next to the cot without picking the infant up), and 'social comforting' (e.g. reading a story). Excessive 'active physical comforting' and reduced 'encourage autonomy' strategy use was associated with infant sleeping problems. Regarding developmental change in strategy between 1 and 2 years, the later the onset at which 'encourage autonomy' became the principal strategy used, the more likely that persistent infant sleeping problems would be present. Factors accounting for the change in strategy use over time were: (i) parental adaptation to infant developmental maturation; (ii) the interaction between maternal cognition and strategy, and, to a lesser extent; (iii) the interaction between infant temperament and parental strategy.

Objective To investigate the effect of partial bladder outlet obstruction on detrusor blood¯ow and oxygen tension (PdetO 2 ) in female pigs. Materials and methods Detrusor-layer oxygen tension and blood¯ow were measured using oxygen-sensitive electrode and radiolabelled microsphere techniques in ®ve female Large White pigs with a partial urethral obstruction and in ®ve sham-operated controls. The effects of chronic outlet obstruction on bladder weight, and cholinergic nerve density and distribution, are also described. Results In the obstructed bladders, blood¯ow and oxygen tension were, respectively, 54.9% and 74.3% of control values at low bladder volume, and 47.5% and 42.5% at cystometric capacity. Detrusor blood¯o w declined by 27.8% and 37.5% in the control and obstructed bladders, respectively, as a result of bladder ®lling, whilst PdetO 2 did not decrease in the controls, but fell by 42.7% in the obstructed bladders. Bladder weight increased whilst cholinergic nerve density decreased in the obstructed animals. Conclusion In pigs with chronic bladder outlet obstruction, blood¯ow and oxygen tension in the detrusor layer were lower than in control animals. In addition, increasing detrusor pressure during ®lling caused signi®cantly greater decreases in blood¯ow and oxygen tension in the obstructed than in the control bladders.