A comparative study on Canine Parvovirus (CPV) infection among the hospitalized dogs at Central Veterinary Hospital (CVH) in Bangladesh and Veterinary College and Research Institute-Madras Veterinary College (VCRI-MVC) in India was conducted during a period of January and July 2015. A total of 270 (80 at CVH and 190 at VCRI-MVC) hospitalized dogs of different breeds were clinically examined. The key clinical signs observed among the CPV infected dogs were bloody diarrhoea (90.4%), vomition (94.5%) and dehydration (severe 85.7%, moderate 10.0% and mild 7.6%). The overall prevalence of CPV infection was higher in VCRI-MVC (42.7%) than CVH (31.2%). The prevalence of CPV was varied significantly (P<0.05) among different age groups, vaccinated and non-vaccinated dogs. Highest prevalence was found 1-3 months (48.7%) old dogs, in compare with 4-6 months (17.2%) and over 6 months (8.3%) old dogs. Highest prevalence was also found in nonvaccinated than vaccinated dogs at CVH, Bangladesh. In VCRI-MVC, India rate of infection also varied significantly (P<0.05) in different age groups (57.4%, 28.9%, 10.0% among 1-3 months, 4-6 months and> 6 months respectively) and 13.2% in vaccinated and 64.4% in non-vaccinated groups. Significant (P<0.05) variation in prevalence of CPV also observed in different breeds-indigenous (50.0%), Spitz (28.2%), Lhasa (18.1%), Doberman (40.0%) and German Shepherd (46.6%).