Objective: To evaluate the efficacy of ultrasound-guided intra-articular injection of triptolide-loaded solid lipid nanoparticle (TP-SLN) for treatment of antigen-induced arthritis (AIA) in rabbits. Material and Methods: Knee joints of 33 New Zealand rabbits with AIA were injected intra-articularly with triptolide (TP: n = 7), TP-SLN (n = 7), betamethasone (BS: n = 7) and dimethyl sulfoxide (DMSO: n = 6). The remaining six rabbits were untreated as the control group. The injection therapy in intervention groups was initiated 1 week after the last immunization in order to avoid irreversible joint damage in the later induction. The ultrasonic scores of the joints were assessed based on synovitis, synovial blood flow and bone erosion. Meanwhile, the correlations of ultrasonic scores and pathological scores were determined. The efficacy and side effects of each group were determined by combining ultrasonic scores, pathological scores, behavior, appetite, weight, joint diameter, skin temperature and biochemical examination.Results: 1) Compared with the control group, the diameters of knee joints of the TP, TP-SLN and BS groups began to reduce 1 week after intra-articular injection (p < 0.01). 2) With the exception of the DMSO group, the interventions were effective in treating synovitis compared with the control group, with TP-SLN and BS being the best. The ultrasonic and pathological scores in synovitis of the TP group were lower than that of model group (Z = -2.726 and -2.530, p < 0.05). The ultrasonic scores differed significantly between BS group and TP-SLN group (Z = −2.17 and -2.360, respectively, p < 0.05) and pathological scores (Z = −2.687 and −2.082, respectively, p < 0.05). 3) Compared with the control group, the TP, BS and TP-SLN were all effective in treating synovial blood flow and bone erosion and there were no significant differences of ultrasonic and pathological scores among them (p > 0.05). The ultrasonic scores of synovial blood flow (Z = −3.033, −2.842, −3.277, p < 0.01) were lower than in the controls. The ultrasonic scores (Z = -2.948, -3.141, -3.210, p < 0.01) and pathological scores (Z = −2.216, −2.505, −2.505, p < 0.05) of bone erosion were also lower than in the model group.4) There were significant correlations between the ultrasonic and pathological scores of synovial inflammation and bone erosion (r = 0.832 and 0.859 respectively, p < 0.001).Conclusions: The therapeutic effect of TP-SLN on arthritis is better than that of TP, but there is no difference between BS and TP-SLN. Therefore, TP-SLN may be used as an alternative to BS in the treatment of rheumatoid arthritis in the future. The ultrasonic and pathological scores showed significant correlation in synovitis and bone erosion. Ultrasound can provide a useful assessment of synovitis in early arthritis.

Potato stem removal is one of the critical technical problems of potato mechanized harvesting; it directly affects the quality of potato harvesting and potato storage. There have been several studies on potato stem removal mechanisms. In practice, however, it was found that the potato stem removal rate was greatly influenced by the posture of the stem before it entered the removal mechanism. In this study, we designed a potato stem posture adjustment mechanism consisting of elastic curtains. A test rig was built to investigate the effect of curtain height, curtain width, and curtain suspension height on potato passage rate and potato stem removal rate. The Box–Behnken design (BBD), combined with the response surface method, was used to conduct the test. The optimal construction and installation parameters for each elastic curtain were determined as 278.93 mm for the curtain height, 20 mm for the curtain width, and 260 mm for the curtain suspension height. The predicted values of potato passage rate and potato stem removal rate under the optimal parameters were 92.36% and 82.83%, which were consistent with the validation test results. Based on the optimization results, a rigid-flexible coupled simulation model for a potato stem transported-posture adjustment process based on Abaqus and Adams was constructed. The maximum impact of the elastic curtain of the stem posture adjustment mechanism on the potato stem was 15.91 N and caused the stem to spring back. The projection angle β′ of the stem posture angle in the xoz plane before posture adjustment was 19.07°, and the β′ of the stem after posture adjustment was 87.18°. At this time, the stem was basically parallel to the rod of the separating sieve and had a high probability of falling from the gap of the bar to complete the removal of the stem. Overall, the stem transport position adjustment mechanism effectively adjusted the stem transported posture and improved the stem removal rate in potato mechanical harvesting.

Background Hypertension is the main risk factor for hemorrhagic stroke. Aldehyde dehydrogenase 2 (ALDH2) may inhibit the occurrence of hypertension by anti-oxidative stress and vascular dilation. The purpose was to investigate the relationship of ALDH2 polymorphisms with hemorrhagic stroke in Hakka Chinese. Methods A total of 329 patients with hemorrhagic stroke and 515 controls were enrolled, and medical records (smoking and drinking history, hypertension, and diabetes) were collected. The genotypes of ALDH2 rs671 of the two groups were detected and analyzed. Results The proportion of the ALDH2 rs671 G/G, G/A, and A/A genotype in patients with hemorrhagic stroke was 55.9%, 37.4%, and 6.7%, respectively, while those were 65.0%, 30.7%, and 4.3% in controls, respectively. There was statistically significant difference in ALDH2 rs671 genotypes distribution ( P =0.021) and alleles distribution ( P =0.005) between patients and controls. Among hemorrhagic stroke patients, no statistically significant differences were observed between patients with ALDH2 different genotypes. Logistic regression analysis showed that there was significantly high risk of hemorrhagic stroke in men (male vs female: adjusted OR 1.711, 95% CI 1.154–2.538, P =0.008), the presence of hypertension (with vs without hypertension: adjusted OR 16.095, 95% CI 10.958–23.641, P <0.001), and the presence of ALDH2 rs671 G/A genotype (G/A vs G/G: adjusted OR 1.679, 95% CI 1.151–2.450, P =0.007) or A/A genotype (A/A vs G/G: adjusted OR 2.516, 95% CI 1.132–5.591, P =0.024). Conclusion ALDH2 rs671 polymorphism likely a risk factor for hemorrhagic stroke.

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