The present study was performed to identify tumor cells in lymph nodes from colorectal adenocarcinomas considered free of disease by the classic hematoxylin-eosin stain, based on the detection of the carcinoembryonic antigen (CEA) and cytokeratins in neoplastic epithelial cells. For this purpose, 603 lymph nodes from 46 lesions were stained by the peroxidase-antiperoxidase technique. Tumor cells were detected in 22 nodes from 12 patients, mainly in the subcapsular sinuses, permitting a restaging of these patients into two groups: those now considered to have metastatic disease and those free of metastases. However, the 5-year follow-up showed no statistical differences in survival between the two groups.

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Using the well known quantum hadrodynamics (QHD), we study the effects of meson-hyperon coupling constants on the onset of hyperons in dense nuclear matter. We use the SU(3) symmetry group to fix all these coupling constants, constrained to experimental nuclear matter results and astrophysical observations. While the discovery of massive pulsars PSR J1614-2230 and PSR J0348+0432 points towards a very stiff equation of state at very large densities, results from heavy ion collisions point in opposite direction for densities below five times the nuclear saturation density. We study some well known parametrizations and see that most of them cannot satisfy both types of constraints. Indeed, although in our model we can simulate a 2.25 M⊙ hyperonic neutron star, the heavy ion collision constraints limit the maximum mass around 2.06 M⊙.

HEADINGS -Rats. Experimental surgery. Physiology.RESUMO -Racional -Milhões de ratos são empregados anualmente em pesquisas e no ensino. A exata relação entre a idade dos ratos, comparada com a idade dos humanos ainda é assunto de discussão e controvérsias. Objetivo -É revisar a literatura, analisando a idade dos ratos em comparação com a idade dos homens. Métodos -Foram revisadas as publicações existentes sobre o assunto contidas nas bases Medline/Pubmed, Scielo, Biblioteca Cochrane e Lilacs cruzando os descritores ratos, cirurgia experimental e fisiologia. Resultados -Ratos desenvolvem rapidamente durante a infância e se tornam sexualmente maduros com cerca de seis semanas de idade, mas atingem a maturidade social cinco a seis meses mais tarde. Na idade adulta, a cada mês do animal é aproximadamente equivalente a 2,5 anos humanos. Vários autores realizaram trabalhos experimentais em ratos e afirmaram existir Correspondence de 30 dias de vida do homem para cada dia de vida do rato. Conclusão -As diferenças na anatomia, fisiologia, desenvolvimento e fenômenos biológicos devem ser levados em consideração quando são analisados os resultados de qualquer pesquisa em ratos em que a idade é um fator crucial. Cuidado especial é necessário ser tomado quando os estudos efetuados pretendem produzir correlação com a vida humana. Para isso, atenção especial é necessária para verificar a fase em dias do animal e sua correlação com os anos em humanos. ABCDDV/836ABSTRACT -Background -Millions of mice are used annually in research and teaching. The exact relationship between age of the animals compared with the age of humans is still subject to discussion and controversy. Objective -Literature review analyzing the age of rats in comparison with men age. Methods -Were reviewed the existing publications on the subject contained in Medline / Pubmed, Scielo, The Cochrane Database of Systematic Reviews and Lilacs crossing the headings rats, experimental surgery and physiology. Results -Rats rapidly develop during childhood and become sexually mature at about six weeks old, but reach social maturity five to six months later. In adulthood, every month of the animal is approximately equivalent to 2.5 human years. Several authors performed experimental studies in rats and estimated 30 days of human life for every day life of the animal. Conclusion -The differences in anatomy, physiology, development and biological phenomena must be taken into consideration when analyzing the results of any research in rats when age is a crucial factor. Special care is necessary to be taken when the intention is to produce correlation with human life. For this, special attention is needed to verify the phase in days of the animal and its correlation with age in years of humans

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Eosinophilia is common in myeloproliferative disorders (MPDs) with abnormalities of chromosome band 5q31-33, including those that present with t(1; 5)(q23;q33). With the development of rational drug therapy, characterization of the molecular targets for these translocations could guide treatment and affect patient survival. We cloned the t(1; 5)(q23;q33) and showed that it fuses platelet-derived growth factor receptor beta (PDGFRB) to the coiled-coil domains of a novel partner protein, myomegalin. Using two-color interphase fluorescence in situ hybridization (FISH), we also demonstrated that the eosinophils are clonal in these disorders. Imatinib mesylate has recently been shown to be efficacious in MPDs with PDGFR activation. Therefore, following our molecular studies, we were able to redirect this patient's treatment. Although she had refractory and progressive disease, once imatinib was started, complete clinical and hematologic remission, as well as major cytogenetic response, was achieved. Given the therapeutic implications, our findings stress the need to aggressively investigate the molecular basis of these diseases, with emphasis on the PDGFR family. IntroductionThe cloning of genes affected by recurrent chromosomal translocations has furthered our understanding of the molecular basis of hematologic malignancies and allowed the development of molecular assays that have improved diagnosis and monitoring of therapy. It has also led to the development of rational targeted drug therapy, including the specific tyrosine kinase inhibitor, imatinib mesylate 1 (Gleevec; Novartis, Basel, Switzerland).Eosinophilia is common in several myeloproliferative disorders (MPDs), notably chronic myeloid leukemia (CML) and the 8p11 myeloproliferative syndrome. 2,3 Less common causes of eosinophilia include MPDs associated with translocations that involve chromosome band 5q31-33. 4 Cloning of these translocations 4-9 showed that they consistently target the PDGFRB gene. In all cases, partner genes contribute oligomerization domains that constitutively activate the tyrosine kinase of plateletderived growth factor receptor beta (PDGFRB). Recently, cryptic deletions of chromosome 4q12 resulting in activation of PDGFRA were found in cases of hypereosinophilic syndrome (HES), 10 suggesting that activation of the PDGFR receptor tyrosine kinase family is a common theme in this phenotypically similar collection of disorders.Of the MPDs with eosinophilia, one unique subgroup presents with a t(1;5)(q23;q33). 11,12 Here, we describe the molecular cloning of this translocation and the clinical/ translational consequences of this finding. Study designAn 11-month-old girl presented with malaise, poor feeding, and hepatosplenomegaly. The initial blood count was hemoglobin (Hb) 60 g/L (6.0 g/dL), white blood cell (WBC) count 43.9 ϫ 10 9 /L with a neutrophilic left shift, marked eosinophilia, monocytosis, and thrombocytopenia. After a bone marrow examination, the diagnosis of an myelodysplastic syndrome (MDS)/MPD syndrome with eosinophilia w...

Preoperative intraarterial (IA) cisplatin (CDP) was administered to 92 patients with nonmetastatic osteosarcoma. The ages of the patients ranged from 4 to 28 years. Sixty-four patients (70%) received 2 or 3 preoperative courses and 28 (30%) received 4 or more. Sixty-two specimens were available for pathologic examination to assess the degree of tumor necrosis. More than 90% tumor destruction was observed in 16 of 42 patients (38%) who received 1 to 3 preoperative courses as opposed to 17 of 20 (85%) who received 4 or more courses. Patients who received 4 or more courses had a 2-fold probability of achieving more than 90% tumor necrosis, and 68% underwent conservative surgery. Of those who received 3 or less courses, 23% underwent conservative surgery. Postoperatively, patients were treated with intravenous (IV) CDP alternating with doxorubicin (ADR) (Adriamycin, Adria Laboratories, Columbus, OH). Pulmonary metastases developed in 36 patients, bone metastases in 2, and local recurrence in 6. Two patients died of cardiac failure without evidence of disease. Thus, 46 patients (50%) were continuously free of disease 18 to 78 months after diagnosis. Univariate and multivariate analyses showed that male sex, low grade preoperative chemotherapy-induced necrosis, and nonosteoblastic histologic condition were prognostic factors predictive of recurrence, while male sex and large tumor size were prognostic factors predictive of death. These results are comparable with those reported by other centers and are superior to our previous experiences that yielded survival rates of 5% to 10%. A substantial number of patients also had the opportunity to achieve tumor removal with conservative surgery.