Gnetum africanum is a twisted woody climbing wild plant i.e.liana found growing naturally in dense equatorial rainforest but currently domesticated in tropical areas of the world. There has been taxonomic confusion in classifying this liana due to its observable diverse morphological characters. Indeed, morphological characterization alone has not offered the needed solution. However, there is dearth of information on the molecular characterization of Gnetum africanum. Morphological and molecular descriptors, could help offers the needed phenotypic and genotypic characterization of this plant much better. This study is aimed at characterizing Gnetum africanum land races to identify species that could be used for the development of high yielding hybrids. Three different land races or accessions of Gnetum africanum was collected from different locations in Nigeria. High quality genomic DNA was isolated from the different samples; it was checked for purity and quantified as appropriate. The DNA samples were subjected to PCR amplification and DNA barcoding studies (chloroplast large subunit of ribulose -1, 5-bisphophate carboxylase rbcL gene). The amplicons were gel eluted, sequenced and checked for homology by using Basic local alignment search tool -BLAST. Identification was obtained from the top similar outcomes of the samples. For Ekim Fig. 1b sample, the forward blast showed 95% similarity with Basella alba voucherID: NC041293.1 and the reverse had 88% similarity with Talinum fruticosum cultivar ID: MK598685.1. For Etinan Fig. 1a sample, the forward blast had 86% similarity with Rhabdothamnussolandri voucher ID: JQ933464.1 and for reverse, there was no similarity founded.
Talinum triangulare (Jacq.) Willd is extensively cultivated vegetable in the agro-ecological zone of the tropical rainforest. It grows to the height of about 10-100 cm tall, bearing small pink flowers, some with white to light purple flowers and with fleshy light green to forest green leaves with swollen roots. It is one of the commonly preferred leafy vegetables in southern Nigeria and Cameroon, where it is used in thickening of soup and as additive in increasing the bulk of stews. It is now attracting considerable interest worldwide as medicinal plant and spice. Previously, we have carried out the morphological characterization of this plant germplasm, where we reported paucity of information on the genetics and molecular characteristics of the plant. This present study aimed at carrying out molecular characterization of different land races of Talinum triangulare germplasm with a view to describing the genetic information / traits of the available land races. The different land races of Talinum triangulare were collected from different part of Nigeria including Uyo, AkwaIbom State bordering Cameroon republic. The research work was carried out at International Institute of Tropical Agriculture Laboratory, IITA, Ibadan, Nigeria. High quality total DNA was isolated from the different Talinumsamples, quantified and cross examined for purity. The genomic DNA samples were treated to PCR amplification using different primers (ribulosebisphosphatecarboxylase rbcL, trnH-psbA intergenic spacer trn H-psbA, maturase K matK, nuclear internal transcribed spacer ITS) and DNAbarcoding technique. Gel electrophoresis was performed and the fragment of the intact DNAi.e. the amplicons was gel eluted, subjected to sequencing and the Basic local alignment search tool BLAST were carry out to verify the sequence homology. Nevertheless, no difference was detected among the Talinum samples, signifying that the land races were genotypically identical and that the phenotypical differences observed were invariably due to environments.
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