Production estimation of citrus rootstock seeds using fruit weightDiversification of rootstocks is crucial, especially because the citrus industry is facing a growing number of diseases affecting orchards. The objective of this study was to determine the number of boxes of citrus needed to obtain a specific mass of seeds. Measurements of fruit weight, fruit number per box, number of seeds per fruit and seed number per box of nine rootstocks were recorded. The maximum number of boxes needed to obtain 1 kg of seeds was 5.43 for the Lemon 'Cravo' and the minimum was 1.01 for genotype Rangpur x Swingle.
Microbial enumeration by serial dilution is one of the best resources to estimate cellular density for microbiological analysis. However, for metataxonomic analysis it is not clear if serially diluted samples may accurately be used for metataxonomic analysis to represent species composition in beef samples. In this study, the effect of sampling preparation of beef samples on the bacterial composition was evaluated by the comparison of dilution and exudate. Based on the obtained results, data obtained from the exudate of the samples were more robust in terms of number of generated reads, but no significant differences in terms of biological diversity were observed (p < 0.05, Wicoxon Test). Besides, both sample preparation procedures evidenced equivalent results of bacterial composition as well as its relative abundances. In conclusion, the use of exudate allows bacterial enumeration and metataxonomic analysis, which is interesting for the point of view of food microbiologists as cellular loads and microbial composition of culturable and unculturable bacteria could be compared.
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