Cancer immunotherapy targeting co-inhibitory pathways by checkpoint blockade shows remarkable efficacy in a variety of cancer types. However, only a minority of patients respond to treatment due to the stochastic heterogeneity of tumor microenvironment (TME). Recent advances in single-cell RNA-seq technologies enabled comprehensive characterization of the immune system heterogeneity in tumors but posed computational challenges on integrating and utilizing the massive published datasets to inform immunotherapy. Here, we present Tumor Immune Single Cell Hub (TISCH, http://tisch.comp-genomics.org), a large-scale curated database that integrates single-cell transcriptomic profiles of nearly 2 million cells from 76 high-quality tumor datasets across 27 cancer types. All the data were uniformly processed with a standardized workflow, including quality control, batch effect removal, clustering, cell-type annotation, malignant cell classification, differential expression analysis and functional enrichment analysis. TISCH provides interactive gene expression visualization across multiple datasets at the single-cell level or cluster level, allowing systematic comparison between different cell-types, patients, tissue origins, treatment and response groups, and even different cancer-types. In summary, TISCH provides a user-friendly interface for systematically visualizing, searching and downloading gene expression atlas in the TME from multiple cancer types, enabling fast, flexible and comprehensive exploration of the TME.

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In order to explore the factors affecting users' behaviors in a free-floating bike sharing (FFBS) system in China, a survey was conducted in Jiangsu province, China in 2017, and the travel characteristics of FFBS users were analyzed. A binary logistic model was applied to quantify the impact of various variables regarding residents' usage preference based on 30401 valid questionnaires. The findings show that (1) FFBS was mainly used for short-distance travel in cities, especially for commuting and schooling, and the time period of travel in FFBS coincided with the rush-hour in urban areas; (2) a higher level of education, a higher daily transportation cost, the convenience of picking up and parking, and the contribution to users' health could promote the usage of FFBS, while malfunctioning bicycles and limited regulations were major obstacles restricting the development of FFBS; (3) interestingly, people with high-incomes rather than those with low-incomes showed an inclination for FFBS owing to the charge mode. This research provides empirical evidence to facilitate the formulation of urban transportation policies and to improve the management of FFBS for the operators.

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