Vaccinia virus with the E3L gene deleted was able to replicate in RK-13 but not HeLa cells. This host range phenotype could be complemented by an E3L gene expressed transiently from a plasmid. Analysis of mutants of E3L indicates that the ability to complement deletion of E3L correlates with the ability of mutated proteins to bind double-stranded RNA but not with their ability to migrate to the nucleus.
From the mechanical complexity in biology. Through history, each century has brought new discoveries and beliefs that have resulted in different perspectives to study life organisms. In this essay, I define three periods: in the first, organisms were studied in the context of their environment, in the second, on the basis of physical and chemical laws, and on the third, systemically. My analysis starts with primitive humans, continues to Aristoteles and Newton, Lamarck and Darwin, the DNA doble helix discovery, and the beginnings of reduccionism in science. I propose that life is paradigmatical, that it obeys physical and chemical laws but cannot be explained by them I review the systemic theory, autopoiesis, discipative structures and non-linear dynamics. I propose that the deterministic, lineal and quantitative paradigm of nature are not the only way to study nature and invite the reader to explore the complexity paradigm. Rev. Biol. Trop. 56 (1): 399-407. Epub 2008 March 31.
Esta publicación presenta una revisión sobre el desarrollo del conocimiento sobrelos organismos vivos a través de la historia y las diferentes perspectivas con que estos se han analizado y definido. Se demuestra como la mayoría de los dogmas del siglo XIX y XXse mantienen a pesar de los nuevos descubrimientos. Esta revisión permite demostrar la importancia de reunir las diferentes disciplinas para lograr una apertura en la definición de los organismos vivos y sus interrelaciones, para lograr cambiar los dogmas existentes. Estosin duda alguna, dará a la investigación en este tema una libertad diferente para entender la naturaleza misma y abrir la posibilidad a la verdadera creatividad y con ella a nuevos descubrimientos que permitan un desarrollo más acorde en el planeta en que vivimos
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