Government subsidy can greatly encourage supply chain enterprises to reduce carbon emissions. To quickly occupy the market, supply chain enterprises form alliances. However, enterprises in the alliance have speculative psychology, and the impact of such free riding behavior on the carbon emissions reduction willingness of supply chain enterprises is still unclear. In this article, government subsidies and free riding behavior parameters are introduced to build a carbon emissions reduction decision model for the government, manufacturers, and suppliers, and the impact of government subsidies and free riding behavior on the decision making of supply chain enterprises is analyzed through evolutionary game theory. The analysis shows that government subsidies have an incentive effect on carbon emissions reduction of supply chain enterprises. After the market stabilizes, even if the government subsidies are gradually withdrawn, the carbon emissions reduction of supply chain enterprises still converges to Pareto optimal equilibrium. The influence of free riding behavior on supply chain enterprises depends on the carbon emissions reduction profit. When the carbon emissions reduction profit is different, the decision of manufacturers and suppliers will be different. The above conclusions provide a reference for governments to strengthen control or enterprises to make decisions on carbon emissions reduction.
Cysteine protease 1 precursor from Zea mays (zmCP1) is classified as a member of the C1A family of peptidases (papain-like cysteine protease) in MEROPS (the Peptidase Database). The 3D structure and substrate specificity of the zmCP1 is still unknown. This study is the first one to build the 3D structure of zmCP1 by computer-assisted homology modeling. In order to determine the substrate specificity of zmCP1, docking study is used for rapid and convenient analysis of large populations of ligand–enzyme complexes. Docking results show that zmCP1 has preference for P1 position and P2 position for Arg and a large hydrophobic residue (such as Phe). Gly147, Gly191, Cys189, and Asp190 are predicted to function as active residues at the S1 subsite, and the S2 subsite contains Leu283, Leu193, Ala259, Met194, and Ala286. SIFt results indicate that Gly144, Arg268, Trp308, and Ser311 play important roles in substrate binding. Then Molecular Mechanics-Poisson-Boltzmann Surface Area (MM-PBSA) method was used to explain the substrate specificity for P1 position of zmCp1. This study provides insights into the molecular basis of zmCP1 activity and substrate specificity.
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