Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine involved in immune response in animals. However, the role of MIFs in plants such as Medicago truncatula, particularly in symbiotic nitrogen fixation, remains unclear. An investigation of M. truncatula—Sinorhizobium meliloti symbiosis revealed that MtMIF3 was mainly expressed in the nitrogen‐fixing zone of the nodules. Silencing MtMIF3 using RNA interference (Ri) technology resulted in increased nodule numbers but higher levels of bacteroid degradation in the infected cells of the nitrogen‐fixing zone, suggesting that premature aging was induced in MtMIF3‐Ri nodules. In agreement with this conclusion, the activities of nitrogenase, superoxide dismutase and catalase were lower than those in controls, but cysteine proteinase activity was increased in nodulated roots at 28 days postinoculation. In contrast, the overexpression of MtMIF3 inhibited nodule senescence. MtMIF3 is localized in the plasma membrane, nucleus, and cytoplasm, where it interacts with methionine sulfoxide reductase B (MsrB), which is also localized in the chloroplasts of tobacco leaf cells. Taken together, these results suggest that MtMIF3 prevents premature nodule aging and protects against oxidation by interacting with MtMsrB.
Hydrogen sulfide (H2S), a novel gas signaling molecule, plays a crucial role in plant growth and stress response. However, little attention has been devoted to the regulation of H2S on nutrient transport and utilization in legume–rhizobia symbiosis systems. Although we have previously proven that H2S synergized with rhizobia to considerably enhance nitrogen (N) metabolism and remobilization in N-deficient soybeans, it remains uncertain if changes in nutrient absorption, metabolism, and accumulation occur concurrently. Therefore, employing a synergistic treatment of H2S and rhizobia, we examined the dry matter biomass and carbon (C), N, phosphorous (P), and potassium (K) nutrient content in various organs of soybean from blooming to maturity. Firstly, H2S and rhizobia application obviously improved leaf and plant phenotypes and biomass accumulation in different organs during N-deficient soybean development. Second, from flowering to maturity, the contents and stoichiometric ratios of C, N, P, and K in various organs of soybean were changed to variable degrees by H2S and rhizobia. Furthermore, H2S collaborated with rhizobia to significantly affect grain nutrient harvest across soybean growth as well as overall plant nutrient accumulation. Consequently, H2S synergizes with rhizobia to optimize grain harvest quality and nutrient accumulation across the plant by managing the rational allocation and dynamic balance of nutrients in diverse organs, hence boosting soybean development and production.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.