A simple and rapid method for Shiga toxin punfication based on specdic bmdmg to the Galcr 1-+4Gal~l-+4Glc globotrioslde tnsaccharlde covalently hnked to polyacryl/polyvmyl (Fractogel) has been developed A cell-free somcate-filtrate of ShrgeNa dysenterzne type 1, stram ll4Sd was passed over the globotrroslde-Fractogel column, and bound toxm eluted with 6 M guamdme-HCl A yield of 36 mg pure toxm/l somcate-filtrate was obtamed, 1 e a one step 1224-fold punficatlon The recovery of blologlcally active toxin was 87% The toxm was purified to homogenaty as shown by native PAGE and SDS-PAGE
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