Soybean hydrolysates and mate tea have been associated with food intake and body weight regulation. The objective of this study was to evaluate the effect of soy hydrolysates and mate tea polyphenols on in vitro adipogenesis using induction of adiponectin as a marker. Swiss 3T3 L1 cell line was incubated with anorectic peptides (AP) and hydrolysates from soy flour (SF), soy concentrate (SC), soy protein isolate (SPI) and soy purified peptides (SPP). Chlorogenic acid (CH) epigallocatechin gallate (EGCG), caffeine (CA) and mate tea (MT, 10 and 50 g dry leaves/L) were also tested. The inhibitory effect on proliferation of preadipocytes and mature adipocytes was analyzed through cell viability and Oil Red O assays. The results indicated strong dose‐response inhibition by AP (IC50 = 6 mg/mL), MT (IC50 = 3.5 mg/ml), NF (IC50 = 2.5 mg/mL) and EGCG (IC50 = 100 μM). SF and CA showed 20% inhibition at 3 mg/mL and 400 μM, respectively. CH (50–300 μM) and SPI (5 mg/ml) did not inhibit preadipocyte proliferation. A significant decrease in number and size of mature adipocytes was observed in SPP treated cells. Soy purified peptides (2000 kDa) and mate tea displayed inhibitory mechanisms on preadipocyte proliferation and on maturation of adipocytes suggesting a potential regulatory role in adipogenesis.
Research Support: Illinois Soybean Association and SAI Company.
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