Candida albicans is a commensal fungus, but circumstantially it may cause superficial infections of the mucous membranes, such as denture stomatitis, when a biofilm is formed on the surface of dental prostheses. This study evaluated the cell viability of C. albicans biofilms against the antifungal activity of thymol when compared with miconazole, by the fluorescence imaging using SYTO 9 and propidium iodide dyes, and counting of colony forming units. C. albicans standard strains (ATCC 11006) were used. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of drugs were determined by broth microdilution tests and the inoculum was standardized to match 0.5 on the McFarland scale (10 6 cfu/mL). Biofilms were grown on the surface of acrylic resin disks in parallel flow chambers from Sabouraud broth supplemented with 10% dextrose. For counting of colony forming units, the fungal solution was sequentially diluted and plated in Sabouraud dextrose agar. Data were analyzed using two-way ANOVA and Tukey's test (a=5%). Biofilms treated with thymol and miconazole presented low numbers of viable cells at the evaluated exposure times. There was statistically significant difference (p<0.05) when compared with control, and the mean value of the exposure times between miconazole and thymol did not differ significantly (p>0.05). In conclusion, both drugs have similar efficiency as antifungal agents against biofilms of C. albicans formed on acrylic surfaces.
Purpose: To evaluate the knowledge of dentists and physicians about the diagnosis and treatment of the patient complaining of halitosis.Methods: Data were collected from a sample of healthcare professionals (81 dentists and 19 physicians) from the city of João Pessoa, Brazil. A structured questionnaire was used to record the professional knowledge about halitosis: frequency, diagnosis, treatment and predisposing factors.Results: Physicians and dentists reported overall different responses based on their clinical knowledge and practice: patient's complaint of halitosis (63% and 38% for physicians and dentists, respectively); halitosis as the main complaint (42% and 23%); perception of halitosis among patients (10% and 67%), professional information to the patient about the halitosis (58% and 89%). Periodontal disease, poor oral hygiene and caries were regarded as the most common causes of halitosis for dentists, but physicians believed that oral cavity changes, sinusitis and reflux disease were the main etiological factors. Conclusion:The results suggest that there is not a high agreement between dentists and physicians regarding halitosis diagnosis and treatment.Key words: Halitosis; dentistry; medicine ResumoObjetivo: Avaliar o conhecimento de dentistas e médicos sobre o diagnóstico e tratamento do paciente com queixa de halitose.Metodologia: Os dados foram coletados em uma amostra de profissionais de saúde (81 dentistas e 19 médicos) na cidade de João Pessoa, Brasil. Um questionário estruturado foi utilizado para registrar o conhecimento profissional clínico sobre halitose: frequência, diagnóstico, tratamento e fatores predisponentes.Resultados: Médicos e dentistas relataram em geral diferentes respostas com base em eu conhecimento e prática clínica: queixa de halitose entre os pacientes (63% e 38% para médicos e dentistas, respectivamente); halitose como queixa principal (42% e 23%); percepção da halitose entre os pacientes (10% e 67%); informação profissional ao paciente sobre a halitose (58% e 89%); realização do tratamento da halitose pelo profissional (68% e 65%). Os dentistas apontaram doença periodontal, higiene bucal deficiente e cárie como as causas comuns de halitose, enquanto que os médicos relataram alterações bucais, sinusite e doença por refluxo como os principais fatores etiológicos.Conclusão: Os resultados sugerem que não há alta concordância entre médicos e dentistas sobre fatores relacionados ao diagnóstico e tratamento da halitose.
Purpose: To evaluate an educative and oral health promotion program for children from three public daycare facilities in the city of João Pessoa (PB), in the Northeastern region of Brazil. Methods: Sample was composed of 219 children who were divided into four age groups: 0 to 12, 13 to 24, 25 to 36 and 37 to 48 months. All children were submitted to clinical exams and preventive procedures of hygiene and topical application of fluoride at 3-month intervals during 12 months. Mothers were given instructions of oral hygiene of their children. The dmft index and the presence of active white spots (AWS) were recorded. Results: Mean (SD) values of the initial and final dmft were 0.65 (1.3) and 0.72 (1.5), respectively (P<0.05). The initial and final numbers of AWS were 44 and 08, respectively. The absence of nocturnal hygiene was associated with AWS for children that received nocturnal breast-feeding. The most relevant variables for dental caries development were: age group, absence of nocturnal oral hygiene and nocturnal breast-feeding; for AWS only the absence of nocturnal oral hygiene was significant (P<0.05). Conclusion: This oral health program showed to be effective in the maintenance of oral health. The most relevant risk factors for dental caries were absence of nocturnal oral hygiene and nocturnal breast-feeding.
There is a risk of cross-contamination by phosphor storage plates used in dental radiology services.
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