ABSTRACT. 5-Fluorouracil (5-FU) is widely used in chemotherapy for treatment of colorectal cancer. Leucine-rich repeat-containing G protein-coupled receptor (LGR) is known to participate in the occurrence and development of breast cancer by regulating the rebirth of tumor vessels. This study aimed to explore the proliferation and apoptosis of HCT116 colorectal cancer cells treated with 5-FU and related molecular mechanisms. 5-FU (20 µg/mL) was used to treat cultured HCT116 cells. An MTT test, flow cytometry, and colony formation assays were used to examine the proliferation and apoptosis of HCT116 cells. Western blotting was applied to detect the expression of the LGR4 protein in HCT116 cells. Small interference RNA or over-expression techniques were used to manipulate LGR4 expression in HCT116 cells and describe the proliferation and apoptosis of HCT116 treated with 5-FU. A dosage of 20 µg/mL 5-FU resulted in a significant decrease in the proliferation and apoptosis of HCT116 cells and significantly decreased expression levels of LGR4. The specific gene silence or over-expression of LGR4 in HCT116 cells increased and decreased the levels of apoptosis in HCT116, respectively. 5-FU induces apoptosis of colorectal cancer cells and inhibits proliferation by suppressing LGR4 proteins.
This study compared the clinical results of surgical treatment of high complex anal fistula with a conventional seton and a new type of seton consisting of an elastic sheath and alloy core tube for irrigation of the wound track. A total of 438 patients were included in the study; 215 were treated with a conventional seton and 223 with the new-type seton. In patients treated with the new-type seton, the wound track was irrigated daily with 100 ml physiological saline, 100 ml hypertonic saline and 0.5 g metronidazole gel from the second postoperative day until seton removal. The new-type seton was associated with significant reductions in recurrence rate, length of stay and healing time compared with the conventional seton, but there were no significant differences in operation time or postoperative incontinence rates. It is concluded that the new-type seton with irrigating tube can improve the treatment of high complex anal fistula.
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