There is increasing evidence that the ∼20 routinely monitored perfluoroalkyl and polyfluoroalkyl substances (PFASs) account for only a fraction of extractable organofluorine (EOF) occurring in the environment. To assess whether PFAS exposure is being underestimated in marine mammals from the Northern Hemisphere, we performed a fluorine mass balance on liver tissues from 11 different species using a combination of targeted PFAS analysis, EOF and total fluorine determination, and suspect screening. Samples were obtained from the east coast United States (US), west and east coast of Greenland, Iceland, and Sweden from 2000 to 2017. Of the 36 target PFASs, perfluorooctane sulfonate (PFOS) dominated in all but one Icelandic and three US samples, where the 7:3 fluorotelomer carboxylic acid (7:3 FTCA) was prevalent. This is the first report of 7:3 FTCA in polar bears (∼1000 ng/g, ww) and cetaceans (<6−190 ng/g, ww). In 18 out of 25 samples, EOF was not significantly greater than fluorine concentrations derived from sum target PFASs. For the remaining 7 samples (mostly from the US east coast), 30−75% of the EOF was unidentified. Suspect screening revealed an additional 37 PFASs (not included in the targeted analysis) bringing the total to 63 detected PFASs from 12 different classes. Overall, these results highlight the importance of a multiplatform approach for accurately characterizing PFAS exposure in marine mammals.
The high proportion of unidentified extractable organofluorine (EOF) observed globally in humans and the environment indicates widespread occurrence of unknown per- and polyfluoroalkyl substances (PFAS). However, efforts to standardize or...
There is increasing evidence that the ~20
routinely monitored per- and polyfluoroalkyl substances (PFASs) account for only
a fraction of extractable organofluorine (EOF) occurring in the environment. To
assess whether PFAS exposure is being underestimated in marine mammals from the
Northern Hemisphere, we performed a fluorine mass balance on liver tissues from
11 different species using a combination of targeted PFAS analysis, EOF and
total fluorine determination, and suspect screening. Samples were obtained from
the east coast United States (US), west and east coast of Greenland, Iceland,
and Sweden from 2000-2017. Of the 36 target PFASs, perfluorooctane sulfonate (PFOS)
dominated in all but one Icelandic and three US samples, where the 7:3
fluorotelomer carboxylic acid (7:3 FTCA) was prevalent. This is the first
report of 7:3 FTCA in polar bears (~1000 ng/g, ww) and cetaceans (<6-190
ng/g, ww). In 18 out of 25 samples, EOF was not
significantly greater than fluorine concentrations derived from sum target
PFASs. For the remaining 7 samples (mostly from the US east coast), 30-75%
of the EOF was unidentified. Suspect screening revealed an additional 33 PFASs
(not included in the targeted analysis) bringing the total to 59 detected PFASs
from 12 different classes. Overall, these results highlight the importance of a
multi-platform approach for accurately characterizing PFAS exposure in marine
mammals.
It is generally accepted that per-and polyfluoroalkyl substances (PFASs) occur primarily in protein-rich tissues such as blood and liver, but few studies have examined the occurrence of legacy and novel PFASs in lipid-rich tissues such as blubber. Here we report the distribution of 24 PFASs, total fluorine, and extractable organic fluorine (EOF) in eight different tissues of a killer whale (Orcinus orca) from East Greenland. The sum of target PFAS concentrations was highest in liver (352 ng/g of wet weight) and decreased in the following order: blood > kidney ≈ lung ≈ ovary > skin ≈ muscle ≈ blubber. Most of the EOF consisted of known PFASs in all tissues except blubber, which displayed the highest concentration of EOF, almost none of which was attributed to targeted PFASs. Suspect screening using high-resolution mass spectrometry revealed the presence of additional PFASs but is unlikely to explain the high concentrations of EOF in blubber. While the identity of this unknown organofluorine and its pervasiveness in marine mammals require further investigation, this work suggests that exposure of killer whales to organofluorine substances may be underestimated by determination of legacy PFASs exclusively in liver or blood.
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