An obligately anaerobic and equol-producing bacterium, designated strain do03 T , was isolated from the caecal content of a rat. Cells were Gram-positive, non-spore-forming rods. The results from a phylogenetic analysis based on 16S rRNA gene sequences showed that strain do03 T formed a separate line of descent in the phylogenetic cluster of the family Coriobacteriaceae. The strain was unable to metabolize glucose or other carbohydrates as sole carbon sources; growth was enhanced in the presence of arginine. The cell wall contained meso-diaminopimelic acid. The major fatty acid was C 18 : 1 cis9 (54.0 %). The strain had one unidentified predominant (91.9 %) quinone that was not menaquinone, methylmenaquinone, demethylmenaquinone, ubiquinone or rhodoquinone. The DNA G+C content was 63 mol%. The data presented in this work show that strain do03T differs from members of the related recognized genera Eggerthella and Denitrobacterium at both the phylogenetic and phenotypic level. Therefore, the strain constitutes a novel genus and species, for which the name Asaccharobacter celatus gen. nov., sp. nov. is proposed. The type strain of the type species is do03The family Coriobacteriaceae was proposed by Stackebrandt et al. (1997) Kageyama & Benno, 2000). A Gram-positive bacterium, designated strain do03 T , capable of converting daidzein to equol via dihydrodaidzein was isolated from the caecal content of a rat (Minamida et al., 2006a). The 16S rRNA gene sequence (1428 bp) of the novel strain showed highest similarity (99 %) with that of the human intestinal bacterium SNUJulong 732 (GenBank accession no. AY310748), which was capable of metabolizing dihydrodaidzein to S-equol (Wang et al., 2005). The levels of similarity with respect to members of the three recognized species of the genus Eggerthella, Eggerthella sinensis HKU14 T (AY321958), Eggerthella hongkongensis HKU10 T (AY288517) and Eggerthella lenta ATCC 25559 T (AF292375), were in the range of 93 to 94 %. On the basis of the phylogenetic analysis, strain do03T differed from members of the genus Eggerthella and belonged to a novel genus of the family Coriobacteriaceae (Minamida et al., 2006a). In this paper, we describe additional taxonomic characteristics of the novel strain in comparison with phylogenetically close genera, and classify it as a novel genus and species within the family Coriobacteriaceae.Cell morphology and size after anaerobic cultivation at 37 u C for 2 days in Gifu anaerobic medium (GAM) broth (Nissui) were examined using phase-contrast microscopy (Nikon). Gram-staining was performed with 3-day-old culture by using a neo-B&M kit (Wako). Colonial morphologies were viewed after cultivation on GAM agar at 37 u C for 2 days in an anaerobic chamber (N 2 /H 2 /CO 2 , 85 : 5 : 10; Coy Laboratory). Biochemical traits were determined using both conventional methods (Holdeman et al., 1977) and the API 50 CH system (bioMérieux). Enzyme activities were analysed with the API ZYM system (bioMérieux) using bacteria harvested from GAM broth supplemented with ...
Requirement for monitoring the state of the elderly in care facilities is increasing year by year and the increase in accidents involving them becomes great concern. In this paper, an ultra-wideband impulse-radio monitoring sensor is suggested which focuses on the bed state most critical to the elderly that need the assistance immediately. Employing our developed state estimation algorithm, various states can be detected that includes sleeping in bed, sitting up in bed and going in and out from the room. The measurements were conducted in a room assumed the care facilities and the performance is investigated where various scenarios are considered. The measurement result shows that the detection rate is more than 91%.
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