Reabsorption of the sodium ion at the renal tubules is the main mechanism for main taining appropriate water content of the body, and it is finely controlled by vasopressin and pituitary-adrenal hormones. Also, the kidney contains an appreciable amount of carbonic anhydrase which catalyzes exchange of H+ with Na+ in the tubules, and diamox, the potent inhibitor of this enzyme, is used as diuretic. This makes it interesting to study on possible regulatory function of vasopressin and pituitary-adrenal system on the carbonic anhydrase activity.Here will be reported our results of experiments on effect of desoxy corticosterone and alrenalectomy on the enzyme activity and diuresis.As substances impeding renal excretion of other drugs competitively, there are caro namide and benemid. They are used clinically as potentiators of penicillin and other drugs.SKF No. 525-A is a recently developed potentiator of mainly centrally acting drugs.They have been also studied as for their action on renal carbonic anhydrase and electrolyte excretion.
METHOD AND MATERIALSMale albino rats were used as animals of experiment. Adrenalectomy was carried out under ether anesthesia, removing both adrenals dorsally. After the operation, 0.9% saline solution -,,-as given ad li.'iturn. To test successfulness of adrenalectomy, the saline solu tion for drinking was exchanged with water for 5 days, and only those which lost over 5 g of body weight were used for the experiment. Success of the operation was further checked by dissection after the experiment.The manometric method of Altschule and Lewis (1) was used for determination of the carbonic anhydrase (CAH) activity. The main compartment of the Warburg vessel received 0.5 ml of 10' homogenate of the fresh kidney with the physiologic saline solu tion, and 1.0 ml of phosphate buffer solution (0.1 M; pH 6.8). The side arm received 1.0 ml of 0.1 M NaHCO, solution, in which a substance to be examined was dissolved. Read ing was taken at the interval of 30 seconds, and the log value of the reading for 210 seconds was plotted on the graph in terms of the time. The tangent value of its line was multiplied with 2.303 to obtain Kc. Ko was determined in the same way with blank solu
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