The shapes of giant unilamellar vesicles (GUVs) enclosing polymer molecules at relatively high concentration, used as a model cytoplasm, significantly differ from those containing only small molecules. Here, we investigated the effects of the molecular weights and concentrations of polymers such as polyethylene glycol (PEG), bovine serum albumin (BSA), and DNA on the morphology of GUVs deflated by osmotic pressure. Although small PEG (MW < 1000) does not alter the mode of shape transformation even at >10% (w/w), PEG with MW > 6000 induces budding and pearling transformation at above 1% (w/w). Larger PEG frequently induced small buddings and tubulation from the membrane of mother GUVs. A similar trend was observed with BSA, indicating that the effect is irrelevant to the chemical nature of polymers. More surprisingly, long strands of DNA (>10 bp) enclosed in GUVs induced budding transformation at concentrations as low as 0.01-0.1% (w/w). We expect that this molecular size dependency arises mainly from the depletion volume effect. Our results showed that curving, budding, and tubulation of lipid membranes, which are ubiquitous in living cells, can result from simple cell-mimics consisting of the membrane and cytosolic macromolecules, but without specific shape-determining proteins.
Deformation of liposomes, or lipid vesicles, has been investigated extensively in terms of the thermodynamic equilibrium of the bending energy of the lipid bilayer membrane. However, the range of such deformation in previous literature has been limited within the moderate surface-to-volume ratio of the vesicles, in which axisymmetric shapes are dominant. Here, we show that neuron-like morphology, in which many lipid tubes extend radially from the mother vesicle, becomes dominant upon the slow osmotic shrinkage of giant unilamellar vesicles (GUVs) initially larger than several tens of micrometers. We show that, in the time-lapse confocal imaging, the emergence of lipid tubes is initiated from the instability that appeared along the annular rim of the flat stomatocyte shape. Since these deformation dynamics into the neuron-like morphology resemble that of the milk-crown formation in liquid splashing, we discuss that the Rayleigh−Plateau capillary instability drives this transformation into a nonaxisymmetric shape.
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