BackgroundMicroRNAs (miRNAs) regulate numerous crucial abiotic stress processes in plants. However, information is limited on their involvement in cadmium (Cd) stress response and tolerance mechanisms in plants, including ramie (Boehmeria nivea L.) that produces a number of economic valuable as an important natural fibre crop and an ideal crop for Cd pollution remediation.ResultsFour small RNA libraries of Cd-stressed and non-stressed leaves and roots of ramie were constructed. Using small RNA-sequencing, 73 novel miRNAs were identified. Genome-wide expression analysis revealed that a set of miRNAs was differentially regulated in response to Cd stress. In silico target prediction identified 426 potential miRNA targets that include several uptake or transport factors for heavy metal ions. The reliability of small RNA sequencing and the relationship between the expression levels of miRNAs and their target genes were confirmed by quantitative PCR (q-PCR). We showed that the expression patterns of miRNAs obtained by q-PCR were consistent with those obtained from small RNA sequencing. Moreover, we demonstrated that the expression of six randomly selected target genes was inversely related to that of their corresponding miRNAs, indicating that the miRNAs regulate Cd stress response in ramie.ConclusionsThis study enriches the number of Cd-responsive miRNAs and lays a foundation for the elucidation of the miRNA-mediated regulatory mechanism in ramie during Cd stress.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1561-5) contains supplementary material, which is available to authorized users.
Panax notoginseng is grown mainly in Yunnan Province. Under the present high-density planting patterns for the plant, to solve the problems of a high rate of seed damage and the inability to use a traditional single air-blowing metering device, this paper designs a six-row air-blowing centralized precision seed-metering device for P. notoginseng to realize mechanized precision seeding of this species. This paper describes the working principle of the seed-metering device, and the main structural parameters are determined by combining theoretical calculations with simulation analysis. A mechanics model of the seed filling, cleaning and pressing processes of the seed-metering device was constructed. The seeds of P. notoginseng in Yunnan Province were selected as experimental subjects. An experimental study on the seed-metering performance of the seed-metering device was carried out using the quadratic rotation orthogonal combination test method. The outlet pressure of the air nozzle, forward velocity and cone angle of the hole were selected as test factors. Mathematical models of the grain spacing qualified index, miss index, multiple index and the coefficient of variation of the row displacement consistency were established to analyze the order of factors affecting indicators. Through parameter optimization, the optimum combination of parameters was determined as follows: the cone angle of the hole is 50°, the forward velocity is less than 0.73 m/s, and the outlet pressure of the air nozzle is 0.32-0.52 kPa. The qualified index of grain spacing is higher than 94%, the miss index is less than 3%, the multiple index is less than 5%, and the coefficient of variation of the row displacement consistency is less than 5%. The test results are essentially consistent with the optimization results. The metering device meets the requirements of precision seeding of P. notoginseng. This study provides a basis for the design of a six-row air-blowing centralized precision seed-metering device for P. notoginseng.
The composition, solution, method and design idea of crop information acquisition device applying internet of things were introduced. The applications of internet of things in crop production research were explained, and several technological difficulties and methods were discussed. Meanwhile, the effect and significance of applying internet of things in relevant disciplines of agricultural production were demonstrated.
The author analyzed the application status of China’s agricultural Internet of Things, conceived and forecast the application of Internet of Things technology in field crop production monitoring, expounded and analyzed the meaning, basic framework and several issues needed to be solved, described that it has an excellent basis and bright prospect for the application of Internet of Things in crop production. Besides, the author also described the significance of applying Internet of Things to this aspect.
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