Nano-carrier systems are highly explored for the slow release of a drug. Chitosan (CS), a polymer, has more applications in nano-drug carrier systems. To explore these applications, currently, we synthesized CS nanoparticles (CS-NPs), and it was allowed to encapsulate free Rutin (RUT) and it resulting in CS-NPs/RUT. Further, it was characterized, and the size of CS-NPs was found to be 79 nm, and upon encapsulating RUT, the particle size was increased to 173 nm, which resulted in CS-NPs/RUT. The entrapment of RUT with CS-NPs was found to be -85%. MTT-based cytotoxicity assay represented the non-lethal nature of CS-NPs/RUT toward normal osteoblast cells, C3H10T1/2 clone8, and showed cytotoxicity against pancreatic adenocarcinoma cells, PANC-1. Furthermore, we extended the study to analyze bacterial growth inhibition and incorporation by CS-encapsulated RUT. It was performed through flow cytometry in different concentrations of 100, 200, and 300 µg/ml of treatments in Escherichia coli. The bacterial inhibition and incorporation of bacteria were significantly higher in CS-NPs/RUT compared with RUT. Hence, the experiments depicted the superiority of CS-NPs/RUT over free RUT that suggested, CS encapsulation as an efficient system for delivering RUT for bacterial growth inhibition and also anti-cancer cell proliferation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.