The germination characteristics of Stellaria media (common chickweed) were investigated over a range of constant temperatures and degrees of moisture stress in order to assess the suitability of hydrothermal time as a basis for modelling germination under field conditions. Maximum percentage germination occurred over a much narrower temperature range around the optimum temperature than previously seen for cultivated crop seed. The entire final percentage germination response to temperature in water was well described by two probit curves, and this model was extended to describe the data at all water potentials at a temperature close to the optimum. The implications of the reduction in germination at nonoptimal temperatures are discussed with respect to the interpretation of germination progress curves and conditional dormancy. After adjusting for maximum percentage germination, a hydrothermal time model was found to fit the data set well within the conditions normally encountered in horticultural seedbeds. This separation of the final percentage germination presents a flexible modelling approach that allows for the different levels of dormancy typically expressed within weed populations. By contrast with many previously reported species, S. media had a synchronous germination rate within the population at any given temperature\water potential combination. This synchronous germination of at least a proportion of the population over a wide range of temperature and water potentials might have ecological significance for the opportunistic germination behaviour of this weed species.
The feasibility of developing a forecasting system for carpogenic germination of Sclerotinia sclerotiorum sclerotia was investigated in the laboratory by determining key relationships among temperature, soil water potential, and carpogenic germination for sclerotia of two S. sclerotiorum isolates. Germination of multiple burials of sclerotia to produce apothecia also was assessed in the field with concurrent recording of environmental data to examine patterns of germination under different fluctuating conditions. Carpogenic germination of sclerotia occurred between 5 and 25 degrees C but only for soil water potentials of >/=-100 kPa for both S. sclerotiorum isolates. Little or no germination occurred at 26 or 29 degrees C. At optimum temperatures of 15 to 20 degrees C, sclerotia buried in soil and placed in illuminated growth cabinets produced stipes after 20 to 27 days and apothecia after 27 to 34 days. Temperature, therefore, had a significant effect on both the rate of germination of sclerotia and the final number germinated. Rate of germination was correlated positively with temperature and final number of sclerotia germinated was related to temperature according to a probit model. Thermal time analysis of field data with constraints for temperature and water potential showed that the mean degree days to 10% germination of sclerotia in 2000 and 2001 was 285 and 279, respecttively, and generally was a good predictor of the observed appearance of apothecia. Neither thermal time nor relationships established in the laboratory could account for a decline in final percentage of germination for sclerotia buried from mid-May compared with earlier burials. Exposure to high temperatures may explain this effect. This, and other factors, require investigation before relationships derived in the laboratory or thermal time can be incorporated into a forecasting system for carpogenic germination.
Three nurseries produced apple rootstocks (M9) and budwood (cv. Royal Gala), which they exchanged at the end of the first year. Each nursery then budded its own budwood onto the rootstocks it had produced and that from the other two nurseries. Budded trees were grown on for a further year before being planted at HRI, East Malling in southern England; NIHPBS, Loughgall in Northern Ireland; and ADAS, Rosemaund in the West Midlands of England. Canker development was monitored twice a year. The position of the infected trees within the orchard was recorded, as was the position of the canker on each tree (main-stem or peripheral). Nectria galligena was isolated from representative cankers and analysed using molecular techniques. At the sites in Northern Ireland and HRI there was a strong positional effect, especially of peripheral cankers, indicating that most of the inoculum was external and had been spread from neighbouring orchards. There was little or no positional effect on main-stem cankers at any of the three sites. The proportions of different isolates taken from peripheral cankers was different in Northern Ireland from that in England, suggesting different populations associated with the geographic areas. In contrast, the populations of N. galligena obtained from main-stem cankers were very similar in England and Northern Ireland. It was concluded that a small proportion of trees developing canker were infected during propagation, with no symptom development until after planting. In a second trial it was demonstrated that trees infected during the propagation phase, and particularly at budding and heading back, could develop canker up to 3 years later. While it is clear that some canker developing in the orchard can be associated with the nursery of production, in climatic conditions conducive to the formation and dissemination of conidia, inoculum from surrounding infected orchards is the primary source of the pathogen. Aerial spread is therefore an essential element of the epidemiology of N. galligena , and its control is a crucial part of any canker-control programme.
Cumulative germination curves were recorded for carrot (Daucus carota L.) seeds at a range of constant temperatures (T ) and water potentials (Ψ) in the laboratory and under variable soil conditions in 15 seed-bed environments in the field. A single base temperature (T b ), a distribution of base water potentials (Ψ b (G)) for percentiles (G) of the population and the hydrothermal time constant (H g ) were determined from laboratory data. Although less effective at low Ψ, it was possible, using these germination parameters, to satisfactorily describe the effect of T and Ψ on germination rates under constant conditions according to the threshold models of thermal and hydrothermal time. These models were applied to field data with the condition that the germination process ceased if T T b for thermal time and additionally Ψ Ψ b (G) for hydrothermal time.Neither model accurately predicted germination patterns in the field. However, the pattern of germination was adequately described in most situations by a modified threshold model in which the predicted progress towards germination was unaffected by soil Ψ, provided it remained above Ψ b (G), and was therefore more rapid under variable seed bed conditions than hydrothermal time. In this modified threshold model, the condition Ψ Ψ b (G) had to be fulfilled at the initiation of radicle extension before germination occurred. This result implies that the initiation of radicle growth operates as a moisture-sensitive step that can determine germination and seedling emergence timing under variable soil-moisture conditions.Seedling emergence was also recorded in the field and used to determine, separately, the impact of germination and post-germination growth on the variation in seedling emergence patterns. The analysis suggests that delays in seedling emergence occur largely in the germination phase, but that seedling losses and variation in the spread of seedling emergence times within the population occur largely during the post-germination growth phase.
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