Epstein-Barr virus (EBV) has been known to be associated genes were subjected to the GCV sensitivity test. Results with many malignant tumors, including nasopharyngeal showed that cells expressing both the LMP1 and the HSVtk carcinoma (NPC). Previous studies have indicated that an genes were highly sensitive to GCV treatment. These cells EBV-encoded oncoprotein, latent membrane protein 1 were introduced into nude mice subcutaneously and (LMP1), is expressed in many NPC tissues. LMP1 has tumors became palpable within 2 weeks. GCV was then been shown to stimulate HIV LTR through the two NF-B introduced intraperitoneally to these mice and the sizes of binding sites within this promoter. In this study, we examthe tumors were measured daily. Results showed that the ined the feasibility of using this property of LMP1 as a tumors regressed in the group of mice carrying cells that therapeutic strategy for the treatment of NPC. This therapy stably expressed both the LMP1 and the HSVtk genes, but consists of the preferential killing of the LMP1-expressing not in mice carrying cells containing LMP1 or HSVtk alone. cells by gene transfer using the NF-B-mediated herpes Our data indicate that the HSVtk gene expressed from a simplex virus thymidine kinase (HSVtk)/ganciclovir (GCV) NF-B-binding motif-containing promoter that is regulated system. The 800-bp HIV-LTR, which contains two NF-B by LMP1 may be used as an in vivo gene therapy strategy binding sites, was used to drive the HSVtk gene. Stable of EBV LMP1-expressing cancers such as NPC. C33A cell clones expressing the LMP1 and the HSVtk
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