Microfluidic platforms use controlled fluid flows to provide physiologically relevant biochemical and biophysical cues to cultured cells in a well-defined and reproducible manner. Undisturbed flows are critical in these systems, and air bubbles entering microfluidic channels can lead to device delamination or cell damage. To prevent bubble entry into microfluidic channels, we report a low-cost, Rapidly Integrated Debubbler (RID) module that is simple to fabricate, inexpensive, and easily combined with existing experimental systems. We demonstrate successful removal of air bubbles spanning three orders of magnitude with a maximum removal rate (dV/dt)max = 1.5 mL min−1, at flow rates required to apply physiological wall shear stress (1–200 dyne cm−2) to mammalian cells cultured in microfluidic channels.
Throwing sports remain a popular pastime and frequent source of musculoskeletal injuries, particularly those involving the shoulder and elbow. Biomechanical analyses of throwing athletes have identified pathomechanic factors that predispose throwers to injury or poor performance. These factors, or key performance indicators, are an ongoing topic of research, with the goals of improved injury prediction, prevention, and rehabilitation. Important key performance indicators in the literature to date include shoulder and elbow torque, shoulder rotation, kinetic chain function (as measured by trunk rotation timing and hip-shoulder separation), and lower-extremity mechanics (including stride characteristics). The current gold standard for biomechanical analysis of the throwing athlete involves marker-based 3dimensional) video motion capture. Emerging technologies such as marker-less motion capture, wearable technology, and machine learning have the potential to further refine our understanding. This review will discuss the biomechanics of throwing, with particular attention to baseball pitching, while also delineating methods of modern throwing analysis, implications for clinical orthopaedic practice, and future areas of research interest. Level of Evidence: V, expert opinion.
Microfluidic platforms use controlled fluid flow to provide physiologically relevant biochemical and biophysical cues to cultured cells in a well-defined and reproducible manner. In these systems, undisturbed flows are critical and air bubbles entering microfluidic channels can result in device delamination or cell damage. To prevent bubble entry, we report a low-cost, Rapidly Integrated Debubbler (RID) module that is simple to fabricate, inexpensive, and easily combined with existing experimental systems. We demonstrate successful removal of air bubbles spanning three orders of magnitude with a maximum removal rate (dV/dt)max = 1.5 mL min -1 , at flow rates corresponding to physiological fluid-induced wall shear stresses (WSS) needed for biophysical stimulation studies on cultured mammalian cell populations.
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