Two populations of HSW, one from Saltillo Coah. which is considered an insecticide-free area and one from Parras, Coah, in which 4-6 treatments are used each yr. The Parras population was collected from an area where 93.8% of the orchards are exposed to Guthion and 93% to Zolone, but have not been exposed to carbaryl for 5 yr. Third instar larvae (avg. wt 15 ±3 mg) were placed in treated vials (35 ml) for exposure to a range of insecticide doses. Doses in series and an acetone check were used or each insecticide with 5 larvae/vial/dose with 4 replicates; vials were held at 25±2°C and a photoperiod of 14:10 (L:D). Mortality was recorded after 24 h, corrected and data were analyzed using probits and the maximum likehood method. LD50 and LD95 values are reported.
The response of PNC from Saltillo and Zaragoza, Coahuila to technical grade Guthion and Sevin was evaluated in laboratory bioassays and compared with the recommended doses of the products. Five larvae ranging in length from 8-10 mm were selected per vial (35 ml) per treatment using 4 replicates. Vials were treated with desired amounts of insecticide dispersed in acetone and an acetone check held at 25±2°C and photoperiod of 14:10 (L:D). Mortality was recorded after 24 h and larvae were considered dead if they failed to respond when touched. Mortality was corrected by Abbott’s formula and data were analyzed using probits and the maximum likehood method.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.