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This study investigated the effect of preceding pro-agility sprints with maximal isometric squats to determine if postactivation potentiation (PAP) could be harnessed in change of direction speed. Sixteen elite under-17 rugby union players (age: 16 ± 0.41yrs; body mass: 88.7 ± 12.1kg, height: 1.83 ± 0.07m) from an Aviva Premiership rugby club were tested. Subjects performed a change of direction specific warm-up, followed by two baseline pro-agility tests. After 10 minutes recovery, 3 x 3-second maximal isometric squats with a 2 minute recovery between sets were completed as a conditioning activity (CA) on a force plate where peak force and mean rate of force development over 300 milliseconds were measured. The pro-agility test was repeated at set time intervals of 1, 3, 5 and 7 minutes following the CA. Overall pro-agility times were significantly slower (p < 0.05) at 1-minute post-CA compared to the baseline (3.3%), with no significant differences occurring at 3, 5 or 7 minutes post-CA. Therefore, it appears that performing multiple sets of maximal isometric squats do not enhance pro-agility performance.