Any new knowledge that goes beyond the stone tools and techniques used in the Palaeolithic and Mesolithic is most significant as it reveals the cultural and technical capabilities of the people living in these periods. In 1963, two pitch finds were discovered in a lignite open-mining pit in the northern foothills of the Harz Mountains, in a layer the geological age of which was dated as being older than 80,000 years. The great significance of these finds was therefore immediately apparent. One of the finds showed a fingerprint as well as the imprints of a flint stone tool and the structure of wood cells. This was indicative of the pitch piece having served as an adhesive to secure a wooden haft to a flint stone blade.Over 30 years later these finds were transferred to the Doerner Institut for investigation. The GC and GC/MS analyses revealed that, in both cases, birch pitches, well-known historical adhesives, had been used. These consist predominantly of pentacyclic triterpenoid components of the lupane type, with betulin forming the major component. The comparison with birch bark extracts showed that the biological peak profile (bio-marker) was surprisingly well preserved in these pitch finds and that hardly any degradation products were present.Today, comparable pitches can easily be produced with modern technical methods, i.e. using airtight laboratory flasks and temperature control facilities. However, any attempt at simulating the conditions of the Neandertal period and at producing these birch pitches without any of these modern facilities will soon be met with many difficulties. This implies that the Neandertals did not come across these pitches by accident but must have produced them with intent. Conscious action is, however, always a clear sign of considerable technical capabilities.

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Prostate-specific membrane antigen (PSMA) is a transmembrane protein expressed in prostate cancer as well as in the neo-vasculature of nonprostatic solid tumors. Here, we determined the expression pattern of PSMA in the vasculature of oral squamous cell carcinoma. Using a previously validated antibody, PSMA staining distribution and cyclooxygenase 2 (COX2) expression status was evaluated in a cohort of patients with squamous cell carcinoma of the oral cavity (n ¼ 96) using immunohistochemistry and was correlated with clinicopathological features as well as outcome. Twenty-four (25%) cases showed no detectable PSMA staining, 48 (50%) demonstrated positive immunoreactivity for PSMA in less than 50% of microvessels and 24 (25%) cases showed strong endothelial PSMA expression in more than 50% of tumor-associated microvessels. High endothelial PSMA expression was associated with greatly reduced survival (18.2 vs 77.3 months; P ¼ 0.0001) and maintained prognostic significance after adjusting for grade and stage in multivariate analysis (hazard ratio ¼ 2.19, P ¼ 0.007). Furthermore, we observed a strong association between endothelial PSMA and cancer cell-specific COX2 expression. In conclusion, we provide the first evidence for the prognostic significance of endothelial PSMA expression in oral squamous cell carcinoma and, suggest a potential interaction between arachidonic acid metabolites and endothelial PSMA expression in the tumor neo-vasculature.

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Unused ancient Egyptian embalming material unearthed at Deir el‐Bahari (c. 1500bc) shed new light on the potential preparation methods for various embalming materials in Pharaonic Egypt. Analyses revealed the presence of phenols, guaiacols, naphthalenes and sesquiterpenoids. These components were attributed to a wood tar oil produced by a dry distillation or smouldering process from the true cedar tree. The preservation effects were elucidated at the molecular level. Bone alkaline phosphatase, an enzyme that is tightly bound in deeper regions of the bone mineral, served as a marker to reveal the efficacy of the investigated compounds. A very similar embalming material was extracted from a Ptolemaic torso (340±170bc), showing powerful bactericidal and fungicidal activity on the mummified tissue and bones. Here, we show that both the unused and the used embalming materials are in striking accordance with Pliny's description of liquid ‘cedrium’, with the abundantly present guaiacol proving the most effective conserving compound.