Three bacterial strains, FP830T, FP2034, and FP2262, were isolated from the rhizosphere soil of rice, corn, and highland barley plants in Beijing, Heilongjiang, and Tibet, respectively, in PR China. These strains are gram-negative, aerobic, and rod-shaped. They exhibited optimal growth at 28°C and pH 7.0 in the presence of 2% (w/v) NaCl and produced a diffusible fluorescent pigment under ultraviolet light when cultured on King's B plates. Phylogenetic analysis based on 16S rRNA gene and multilocus sequence data showed that FP830T, FP2034, and FP2262 belonged to the genus Pseudomonas and formed a distinct branch. The genome sizes of these three isolates ranged from 6.4 to 6.6 Mbp with a G+C content of 60.86-61.02 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization values of strains FP830T, FP2034, and FP2262, along with their closest relatives, were not higher than 92.7% and 49.8%, respectively. These values were lower than the threshold range for prokaryotic species delineation. The dominant cellular fatty acids (> 10%) were C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and summed feature 8 (C18:1 ω7c). The major polar lipids detected were phosphatidylethanolamine, diphosphatidylglycerol, and aminophospholipids. Additionally, the predominant respiratory quinone was ubiquinone (Q-9). Based on phenotypic, chemotaxonomic, and genotypic data, it was concluded that strains FP830T, FP2034, and FP2262 represented a novel species within the genus Pseudomonas. Therefore, the name Pseudomonas beijingensis sp. nov. was proposed for this novel species. The type strain was FP830T (=ACCC 62448T = JCM 35689T).