2D nanomaterials have attracted considerable research interest in drug delivery systems, owing to their intriguing quantum size and surface effect. Herein, Gd -doped monolayered-double-hydroxide (MLDH) nanosheets are prepared via a facile bottom-up synthesis method, with a precisely controlled composition and uniform morphology. MLDH nanosheets as drug carrier are demonstrated in coloading of doxorubicin and indocyanine green (DOX&ICG), with an ultrahigh drug loading content (LC) of 797.36% and an encapsulation efficiency (EE) of 99.67%. This is, as far as it is known, the highest LC level at nearly 100% of EE among previously reported 2D drug delivery systems so far. Interestingly, the as-prepared DOX&ICG/MLDH composite material shows both pH-controlled and near-infrared-irradiation-induced DOX release, which holds a promise in stimulated drug release. An in vivo dual-mode imaging, including near-infrared fluorescence and magnetic resonance imaging, enables a noninvasive visualization of distribution profiles at the tumor site. In addition, in vitro and in vivo therapeutic evaluations demonstrate an excellent trimode synergetic anticancer activity and superior biocompatibility of DOX&ICG/MLDH. Therefore, MLDH nanosheets provide new perspectives in the design of multifunctional nanomedicine, which shows promising applications in controlled drug delivery and cancer theranostics.

Bioassay-guided fractionation of the 60% ethanol extract of the stems of Dendrobium nobile using the DPPH assay led to the isolation of two new bibenzyl derivatives, nobilin D (1) and nobilin E (2), and a new fluorenone, nobilone (3), together with seven known compounds (4-10). Their structures were determined on the basis of spectroscopic analyses. Compounds 1, 2, 4, 7, 8, and 10 exhibited significant antioxidant activity higher than or equivalent to vitamin C in the DPPH assay, and compounds 1, 3, 4, and 7-10 displayed higher antioxidant activity than vitamin C in the ORAC assay. Compounds 1, 2, and 10 also exhibited stronger inhibitory effects on NO production than resveratrol.

The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a powerful gene editing tool and got a broad application in genome research field due to its ease of use and cost-effectiveness. The performance of CRISPR/Cas relies on well-designed single-guide RNA (sgRNA), so a lot of bioinformatic tools have been developed to assist the design of highly active and specific sgRNA. These tools vary in design specifications, parameters, genomes and so on. To help researchers to choose their proper tools, we reviewed various sgRNA design tools, mainly focusing on their on-target efficiency prediction model and off-target detection algorithm.

The Wyner model has been widely used to model and analyze cellular networks due to its simplicity and analytical tractability. Its key aspects include fixed user locations and the deterministic and homogeneous interference intensity. While clearly a significant simplification of a real cellular system, which has random user locations and interference levels that vary by several orders of magnitude over a cell, a common presumption by theorists is that the Wyner model nevertheless captures the essential aspects of cellular interactions. But is this true? To answer this question, we consider both uplink and downlink transmissions, and both outage-based and average-based metrics. For the uplink, for both metrics, we conclude that the Wyner model is in fact quite accurate for systems with a sufficient number of simultaneous users, e.g. CDMA. Conversely, it is broadly inaccurate otherwise. With multicell processing, intracell TDMA is shown to be suboptimal in terms of average throughput, in sharp contrast to predictions using the Wyner model. Turning to the downlink, the Wyner model is highly inaccurate for outage since it depends largely on the user locations. However, for average or sum throughput, the Wyner model serves as an acceptable simplification in certain special cases if the interference parameter is set appropriately.