RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.
We describe the draft genome of the microcrustacean Daphnia pulex, which is only 200 Mb and contains at least 30,907 genes. The high gene count is a consequence of an elevated rate of gene duplication resulting in tandem gene clusters. More than 1/3 of Daphnia’s genes have no detectable homologs in any other available proteome, and the most amplified gene families are specific to the Daphnia lineage. The co-expansion of gene families interacting within metabolic pathways suggests that the maintenance of duplicated genes is not random, and the analysis of gene expression under different environmental conditions reveals that numerous paralogs acquire divergent expression patterns soon after duplication. Daphnia-specific genes – including many additional loci within sequenced regions that are otherwise devoid of annotations – are the most responsive genes to ecological challenges.
In order to obtain a systems-level understanding of a complex biological system, detailed
proteome information is essential. Despite great progress in proteomics technologies, thorough
interrogation of the proteome from quantity-limited biological samples is hampered by inefficiencies
during processing. To address these challenges, here we introduce a novel protocol using
paramagnetic beads, termed Single-Pot Solid-Phase-enhanced Sample Preparation (SP3). SP3 provides a
rapid and unbiased means of proteomic sample preparation in a single tube that facilitates
ultrasensitive analysis by outperforming existing protocols in terms of efficiency, scalability,
speed, throughput, and flexibility. To illustrate these benefits, characterization of 1,000 HeLa
cells and single Drosophila embryos is used to establish that SP3 provides an
enhanced platform for profiling proteomes derived from sub-microgram amounts of material. These data
present a first view of developmental stage-specific proteome dynamics in
Drosophila at a single-embryo resolution, permitting characterization of
inter-individual expression variation. Together, the findings of this work position SP3 as a
superior protocol that facilitates exciting new directions in multiple areas of proteomics ranging
from developmental biology to clinical applications.
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