[14C] Cholesterol-5 alpha, 6 alpha-expoxide, administered to mice by either gastric intubation or skin painting, was rapidly and primarily excreted in the feces. Residual amonts of the epoxide and its metabolites were found in a wide variety of organs, and persisted for at least 72 hr. At some sites (principally the liver, the small intestinal contents and the combined stomach/duodenum and their contents), the labeled compound existed in a water-soluble form which could not be extracted with chloroform/methanol. Treatment of the small intestinal contents with a preparation of beta-glucuronidase/sulfatase produced a marked increase in the amount of organic-solvent-extractable cholesterol-alpha-epoxide and other polar metabolites. Unchanged epoxide was found mainly in the feces and the skin at the site of application. On the basis of these results, stool specimens, and not blood samples, should be analyzed to detect the presence of this compound and/or its metabolites in vivo.
The Syrian golden hamster flank organ, composed of clusters of large sebaceous glands, has been used to investigate effects of systemically or topically applied hormones, antiandrogens, and retinoids. Accurate assessments of flank organ sebaceous gland volume and its correlation with in vivo measurements of flank organ size have not been reported. Sixty flank organs were examined. In vivo flank organ size was expressed as the product of the greatest longitudinal (DL) and greatest transverse (DT) diameters of the palpable bulk. Sebaceous gland volumes were calculated from planimetric analyses of serially sectioned specimens of flank organs using a computer-assisted planimetry system. In vivo measurements (DL X DT) were found to correlate with planimetrically determined flank organ volume. Planimetric analysis of every 10th section of a serially sectioned flank organ was almost as sensitive as analysis of every section in determining sebaceous gland volume. Computer-assisted planimetry of serially sectioned flank organs was found to be an accurate and reproducible method for assessing gland volume. In vivo measurements of the palpable bulk are a good indicator of sebaceous gland size and correlate well with planimetrically determined sebaceous gland volumes.
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