SUMMARYThe evaluation of the hemagglutination titer is an option to evaluate the response of acquired immune system in order to assess the immunocompetence for antibodies production. The study was carried out to standardize the antibody titer against rabbit red blood cell in immunized fish and fed diets with levamisole (0; 250 and 500 mg.kg -1 levamisole). As a result, the cell cluster agglutination can be observed by the naked eye. Fish fed 250 mg.kg -1 of levamisole have shown the highest hemagglutination antibodies titer; however, fish fed 500 mg.kg -1 of levamisole have revealed titers equivalent to control group that was fed levamisole-free diet. This study has validated the methodology for determination of hemagglutination antibody titer of immunized fish and has found that antibody titers increased after feeding a diet containing 250 mg.kg -1 of levamisole during 10 days.
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