The paper deals with laboratory testing of PAHs biodegradation in samples of sleeper subsoil via two chosen microorganisms, bacteria Pseudomonas monteilii and Rhodococcus degradans. The obtained results show that the pure bacterial culture R. degradans removed 46 % PAHs in 28 days. Bacterium P. monteilii was far more successful in degrading PAHs, it managed to remove 74 % PAHs. The worst result was from the mixture of both bacteria which degraded only 34 % PAHs.
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