We report a focus shaping technique using generalized cylindrical vector beams. A generalized cylindrical vector beam can be decomposed into radially polarized and azimuthally polarized components. Such a generalized cylindrical beam can be generated from a radially polarized or an azimuthally polarized light using a two-half-wave-plate polarization rotator. The intensity pattern at the focus can be tailored by appropriately adjusting the rotation angle. Peak-centered, donut and flattop focal shapes can be obtained using this technique.

We report the development of a modular and optimized thin-sheet laser imaging microscope (TSLIM) for nondestructive optical sectioning of organisms and thick tissues such as the mouse cochlea, zebrafish brain/inner ear, and rat brain at a resolution that is comparable to wide-field fluorescence microscopy. TSLIM optically sections tissue using a thin sheet of light by inducing a plane of fluorescence in transparent or fixed and cleared tissues. Moving the specimen through the thinnest portion of the light sheet and stitching these image columns together results in optimal resolution and focus across the width of a large specimen. Dual light sheets and aberration-corrected objectives provide uniform section illumination and reduce absorption artifacts that are common in light-sheet microscopy. Construction details are provided for duplication of a TSLIM device by other investigators in order to encourage further use and development of this important technology.

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We report on a novel microellipsometer that uses a spatially filtered high-numerical-aperture (NA) lens for large-angle ellipsometric illumination and high spatial resolution. A radially symmetric ellipsometric signal is achieved with two half-wave plates to produce a pure polarization rotation and a birefringent cube as a radial analyzer. This radial symmetry offers a better signal-to-noise ratio compared with other microellipsometer techniques. Ellipsometric measurement with a spatial resolution of 0.5 microm is performed with a He-Ne (632.8-nm) laser source and an objective lens with an NA of 0.8. Experimental data on SiO2 samples with different thicknesses are in good agreement with spectroscopic ellipsometer results. We acquired ellipsometric images of photoresist microstructure through scanning the sample. Surface profiles of the photoresist microstructure are derived from the ellipsometric data and compared with the results from a stylus profiler.

We describe a phase plate based on the fractional-Talbot effect that converts a single expanded laser beam into a regular array of uniformly illuminated apertures with virtually 100% efficiency. The size, spacing, and fill factor of the illuminated aperture grid can be freely chosen to interface with a variety of electro-optic devices. A binaryoptics phase plate is demonstrated that converts uniform illumination into an array of square illumination cells with a fill factor of 1/16.