1. The mechanism of the anti-nutritive activities of soluble non-starch polysaccharides (NSPs) in broiler diets was investigated with emphasis on the inter-relationship between viscosity and fermentation along the gut. Isolated soluble NSP were added to a control diet to effect high gut viscosity, and in vivo depolymerisation of the NSP was achieved using a commercial glycanase. 2. Addition of soluble NSPs significantly (P < 0.01) increased gut viscosity, reduced the AME of the diet and depressed the growth and FCE of the birds. Enzyme supplementation of the NSP-enriched diet reversed the adverse effects, increasing (P < 0.01) weight gain, FCE and AME. Comparisons of the viscosities (mPa) in birds fed on the NSP-enriched diet and the same diet supplemented with enzyme were respectively: 11.9 v. 2.3 in the duodenum; 78.3 v. 4.4 in the jejunum and 409.3 v. 10.8 in the ileum. 3. Caecal volatile fatty acid concentration was markedly (P < 0.01) elevated by enzyme supplementation, whereas ileal fermentation was inhibited. 4. Microscopic examination revealed that, among birds fed on the NSP-enriched diet, there had been extensive small intestinal fermentation, which was eliminated by the enzyme supplementation. 5. Addition of a synthetic antibiotic (Amoxil) had no beneficial effects. 6. The current study demonstrated that increased fermentation occurs in the small intestine when a large amount of viscous NSPs is present in the diet and this is detrimental to the performance and well-being of poultry.
Quantitative trait gene or locus (QTL) mapping is routinely used in genetic analysis of complex traits. Especially in practical breeding programs, questions remain such as how large a population and what level of marker density are needed to detect QTLs that are useful to breeders, and how likely it is that the target QTL will be detected with the data set in hand. Some answers can be found in studies on conventional interval mapping (IM). However, it is not clear whether the conclusions obtained from IM are the same as those obtained using other methods. Inclusive composite interval mapping (ICIM) is a useful step forward that highlights the importance of model selection and interval testing in QTL linkage mapping. In this study, we investigate the statistical properties of ICIM compared with IM through simulation. Results indicate that IM is less responsive to marker density and population size (PS). The increase in marker density helps ICIM identify independent QTLs explaining 45% of phenotypic variance. When PS is 4200, ICIM achieves unbiased estimations of QTL position and effect. For smaller PS, there is a tendency for the QTL to be located toward the center of the chromosome, with its effect overestimated. The use of dense markers makes linked QTL isolated by empty marker intervals and thus improves mapping efficiency. However, only large-sized populations can take advantage of densely distributed markers. These findings are different from those previously found in IM, indicating great improvements with ICIM.
Canine parvovirus (CPV) is highly contagious and is a major cause of haemorrhagic enteritis and myocarditis in dogs. We investigated the genetic variation of emerging CPV strains by sequencing 64 CPV VP2 genes from 216 clinical samples of dogs from Heilongjiang, Jilin, Liaoning, Shandong and Hebei in 2014. Genetic analysis revealed that CPV-2b was predominant in Hebei and CPV-2a was predominant in the other four provinces. In addition, a CPV-2c strain has emerged in Shandong province. All samples had a Ser-Ala substitution at residue 297 and an Ile-Arg substitution at residue 324. Interestingly, in five separate canine samples, we found a mutation of Gln370 to Arg, until now detected only in isolates from pandas. The phylogenetic analysis showed clear distinctions between epidemic isolates and vaccine strains and between Chinese CPV-2c strains and CPV-2c strains found in other countries. Monitoring recent incidence of CPV strains enables evaluation and implementation of disease control strategies.
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